| Glioma is one of the most common central nervous system tumors. The incidence of glioma is high and the treatment efficacy has not been satisfactory. Better prognosis of glioma has been achieved with the improvement of surgical technique and the widespread application of chemotherapy and radiotherapy, however the overall prognosis of most gliomas is still poor. Oligodendroglioma(OG) is one major type of gliomas, which accounts for2-5%of primary brain neoplasms and4-15%of gliomas. Because these tumors respond well to chemotherapy and have good prognosis, the reason for this is becoming a hot topic in glioma research. It was reported that response to chemotherapy and good prognosis of OG patients were closely related to the presence of allelic losses on lp(or1p and19q) in the resected tumor tissue. Further research revealed that OGs with or without1p LOH had profound differences, such as tumor location, response to radiotherapy, prognosis. So the two types of OGs undistinguishable under optical microscopy may be tumors with different molecular biological character. The molecular classification of OGs may be helpful to their diagnosis and therapy. Except for the difference of lp/19q LOH, other features, such as expression of genes, proteins, and molecular biological mechanisms, are not clearly defined for the OGs. Further studies are needed to elucidate the molecular signatures, and thus possible mechanisms of OGs with1p LOH.Immunohistochemistry is one of the most common pathology diagnosis method, which is easy to operate and cheap cost. It can achieve semi-quantitative detection depending on the situation of intracellular staining. We use immunohistochemistry to semi-quantitatively detect the proteins in OG with and without1p LOH, discuss the correlation between differential proteins and prognosis of patients.The physical condition, activity ability and medical requirement of OG patients is usually estimate by Karnofsky Performance Scale (KPS). It is divided into11grades depending on the physical condition of the patient, from100(no symptom or clinical sign) to0(death). KPS is considered to be one of the most common used scale to estimate the condition of patient since inception in1948. It is generally acknowledged that the patients whose KPS is more than60can tolerant normaltherapeutic schedule and be included into clinical trials. We follow up the patients who has recepted OG resection, estimate the condition by KPS and discuss the correlation between differential proteins and prognosis of patients Part I:The expression of HMGB1, MAP2, UBA1, ATP6V1E1in OGs with different1p LOH and its significanceObjective:To examine the expression of HMGB1, MAP2, UBA1, ATP6V1E1in OGs tissues with different1p LOH.Methods:The expression of HMGB1, MAP2, UBA1, ATP6V1E1were validated in44independent OGs tissue samples with different1p LOH status using IHC method.Results:The result of IHC validated that the expression of HMGB1, MAP2and ATP6V1E1was higher in OGs with1p LOH than in OGs without1p LOH, while the expression of UBA1was higher in OGs without1p LOH than in OGs with1p LOH.Conclusion:The results of IHC show the difference in the expression of proteins in OGs tissues with different1p LOH.These specific proteins may be useful in diagnosis, prognosis and treatment selection for OG patients.Part Ⅱ:Correlation analysis between MAP2, ATP6V1E1and prognosis of oligodendroglioma patientsObjective:To discuss the correlation between MAP2, ATP6V1E1and prognosis of patients.Methods:33OG patients was followed-up with KPS score, compared the change of KPS score with IHC score of MAP2and ATP6V1E1, and discuss the correlation.Results:The change of KPS score was significantly related to the IHC score of MAP2and ATP6V1E1. The correlation was further improve after we combined the IHC scores of these two proteins.Conclusion:MAP2and ATP6V1E1may be related to sensitivity of chemotherapy and the prognosis. It can be used in judgment of prognosis, the direction of therapy, and further discussion of pathogenesis. |
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