Study On The Correlation Between Nogo-a Expression And Secondary Epilepsy Caused By Oligodendroglioma

Chapter I Epilepsy incidence of different pathologicalclassi-fication gliomasObjective To investigate the epilepsy incidence (EI) of different pa-thological classification gliomas.Methods Medical records of 329 pati-ents with glioma were studied. Results The El of astrocytoma was 25.9%, low-grade astrocytoma (WHOI-Ⅱ) got an EI of 34.8%,the high-grade ast-rocytoma (WHOⅢ-Ⅳ) got an EI of 16.5%. The EI of oligodendroglioma was 39.2%,including the low-grade oligodendroglioma (WHOⅡ) with an EI of 46. 2%, and the high-grade oligodendroglioma (WHO Ⅲ) with an El of 16.7%. The EI of glioblastoma、ependymoma and gangliocytoma were 16.7%、0 and 46.2%, the EI of mixed glioma was 62.5%, and the El of other types glioma was 3 0%. The EI of oligodendroglioma was remarkably higher than that of the g-lioblastoma(P<0.05), and compared with the El of astrocytoma, the EI of o-ligodendroglioma were higher,but there was no significant difference (P> 0.05).the El of astrocytoma was higer than that of glioblastoma,but no remarkable difference (P>0.05). The EI of oligodendroglioma (WHO Ⅱ) in t-he frontal or/and temporal lobe was higher than that of astrocytoma (WHO Ⅱ) in the frontal or/and temporal lobe, while there is no significant difference between them (P>0.05).Conclusion The oligodendroglioma got a high EI among that of the different pathological classification gliom-as.ChapterⅡ Expression of Nogo-A protein in oligodendroglioma tissues in epilepsy group and nonepilepsy groupObjective To investigate the expression of Nogo-A protein in oligoden-droglioma tissus in epilepsy group and nonepilepsy group of oligodendro-glioma. Methods A total of 23 patients with oligodendroglioma were div-ided into epilepsy group and nonepilepsy group,Nogo-A expression were d-etested by Immunohistochemistry and Westernblotting. The comparison of I-mmunoreactivity score (IRS) and Nogo-A/GAPDH were made between epilepsy group and nonepilepsy group. Results Immunoreactivity score (IRS) of Nogo-A expression in the epilepsy group was (6.79±2.00), which was remarkab-ly increased compared with the IRS of nonepilepsy group(4.44±1.59)(P<0. 05). Westernblotting analysis showed that Nogo-A/GAPDH of the epilepsy g-roup was(0.814±0.217), which was significantly increased compared with that of the nonepilepsy group(0.540±0.153) (P<0.05). Conclusion The exp-ression of Nogo-A in oligodendroglioma tissus of epilepsy group was sig-nificantly increased compared with that of nonepilepsy group, there may be a correlation between the Nogo-A expression and oligodendroglioma-cau sed epilepsy.Chapter Ⅲ Effect of Nogo-A on calcium concentration in new-born rat cortical neurons cultured in vitroObjective To investigate the effect of Nogo-A on the calcium concentr-ation in new-born rat cortical neurons cultured in vitro. Methods By u-sing microdissection-enzyme digestion method, cortical neurons of the new-born rats were extracted, and then the neurons cultured in vitro were i-dentified. By different interference conditions,the neurons were divided into four groups:normal control group (krebs-ringer group), Nogo-A group, Nogo-A+Nimodipine group and Nimodipine group. By using laser scanning co-nfocal microscope, neuronal calcium signals marked by F1-uo4/AM were tes-ted at different time points. Results After drug administration, the ca-lcium signals of control group and Nimodipine group had no significant change at all time points(P>0.05), the calcium signals of Nogo-A group a-nd Nogo-A+Nimodipine group were remarkably increased over time(P<0.05). At the time point of 1 min, there was no significant difference in calc-ium signals between the four groups(P>0.05). at the time points of 3、5、1 0 min, the calcium signals of Nimodipine group had no significant change compared with that of control group(P>0.05), while the calcium signals of Nogo-A group and Nogo-A+Nimodipine group were significantly increased c-ompared with that of control group and Nimodipine group(P<0.05). Conclu-sion Nogo-A protein could contribute to the calcium overload of corti-cal neurons of new-born rats cultured in vitro.