IPS Cells Reprogrammed From Human Hair Follicle Mesenchymal Stem Cells

Reprogramming of somatic cells into inducible pluripotent stem cells (iPSCs) providesan alternative to using embryonic stem cells (ESCs). Therefore, finding a kind of cells whichare easily accessible, methods for their retrieval reproducible, and the harvesting procedurerelatively noninvasive is the key for obtaining a great quantities of iPSCs. Because of humanhair follicle mesenchymal stem cells (hHF-MSCs) are very easy to procure, abundancy insources, and have no injury to the body get great extensive attention in this territory. Whetherthese hHF-MSCs can be reprogrammed has not been previously reported and naturally tobecome our next goal. Once we have induced successfully, it represents a promising resourceto take place of hESCs to study in the research of regenerative medicine. At the same time,this would provide a new source of reprogrammable target cells for research intopersonalized regenerative medicine.A major goal of stem cell research is the use of cells obtained from patient-derivedpluripotent cells for therapeutic purposes. ESCs are the most primitive stem cells.They arederived from pre-implantation embryos and can differentiated into all three embryonic germlayers. The characterization of ESCs as totipotent has made them a powerful tool forregenerative medicine. However, hESCs-based therapeutic research is technicallychallenging and further complicated by ethical and legal concerns, significantly limiting itspotential. Therefore, it has been the goal of the scientific community to explore new andinnovative approaches to obtain hESCs-like cells that do not destroy human embryos.At present, a wide variety of cells from many different species, including humans, havebeen reprogrammed into iPSCs. However, the methods for obtaining target cells forreprogramming are invasive and painful, often accompanied by restrictions on time, placeand quantity of available tissues. In our study we attempted to establish a simple, universallyapplicable tissues and noninvasive sampling method. We also sought to determine whetherhHF-MSCs represent a reprogrammable cell type for use in generating iPSCs.In this experiment, hHF-MSCs were isolated from hair follicle tissue, and confirmedtheir mesenchymal nature by detecting cell surface antigens and multilineage differentiationpotential towards to adipocytes, osteoblasts and chondroblasts. Then hHF-MSCs werereprogrammed into iPS cells by lentiviral transduction with Oct4, Sox2, c-Myc, Klf4.hHF-MSC-derived iPS cells appear indistinguishable from hESCs in colony morphology, expression of alkaline phosphatase, specific hESCs surface markers, SSEA-4, SSEA-3,Tra-1-60, Tra-1-81, Oct4, Nanog and E-Cadherin and endogenous pluripotent genes, wheninjected into NOD-SCID mice, hHF-MSC-derived iPS cells formed teratomas consisting ofthree germ layers of endoderm, mesoderm and ectoderm.In conclusion, MSCs were isolated from human hair follicle tissues by noninvasivemeans. The differentiation potential of hHF-MSCs was increased by reprogramming withYamanaka factors. The method used to access hHF-MSCs was very convenient and enabledquick establishment of a large-scale hHF-MSCs library, which was then used to create anhHF-MSC-derived iPSCs bank. This work lays the foundation for further research into howthese hHF-MSC-derived iPSCs can be applied to personalized regenerative medicine.