The Experimental And Clinical Research Of Bone Marrow Stem Cells Transplantation For The Treatment Of The Lower Limb Ischemic Diseases After The Bone Marrow Stimulation

Objectives:We observed the effectiveness of bone marrow stem cells transplantation to treat the limb ischemic diseases after the bone marrow stimulation and researched the mechanism of bone marrow stem cells transplantation to treat the limb ischemic diseases after the bone marrow stimulation.Methods:Three parts were consisted of this research. The part 1 was focus on the experimental research of bone marrow stem cells transplantation to treat the limb ischemic disease after the bone marrow stimulation. In this part, the effectiveness of bone marrow stem cells transplantation on the animal models with the bone marrow stimulation were compared with the animal models without the bone marrow stimulation. The results of this part provided the theoretic basis for the clinical research. The part 2 and the part 3 were the clinical researches of bone marrow stem cells transplantation for the treatment of Arteriosclerosis Obliterans(ASO) and Thromboangiitis Obliterans(TAO) after the bone marrow stimulation respectively.Results:Experimental research:1 The number of migrating and adhering cells in the bone marrow stimulating group were significantly increased in comparison with the non-stimulating group.2 The number of collateral branches of the vessel, CD31 positive cells and the expressing level of VEGFmRNA in the bone marrow stimulating group were significantly increased in comparison with the non-stimulating group.Clinical research: 1 The number of Bone Marrow-Mononuclear Cells (BM-MNCs) in the bone marrow stimulating group was significantly higher than it in the non-stimulating group under obtaining the similar volume of the bone marrow blood.2 The percent of CD34 positive cells in the bone marrow stimulating group was significantly higher than it in the non-stimulating group3 The symptoms and the part of objective indexs in the bone marrow stimulating group were significantly improved in comparison with the non-stimulating group.Conclusions:1 The capabilities of revascularization after the bone marrow stem cells transplantation in the bone marrow stimulating group were obviously superior to the capabilities of revascularization in the non-stimulating group for the treatment of ASO and TAO.2 The mechanisms of bone marrow stem cells transplantation after the bone marrow stimulation for the treatment of the lower limb ischemia may be the increasing of endothelial progenitor cells’(EPCs)number and the improvement of EPCs’functions.3、Bone marrow stem cells transplantation after the bone marrow stimulation is a supplement and optimization of the traditional bone marrow stem cells transplantation for the treatment of the limb ischemic diseases.