Study On The Radiation Damage Of Growth And The Protection In Caenorhabditis Elegans

Objective: To establish the model of radiation damage by 60Coγ-ray in Caenorhabditis elegan; investigate the radiation damage of growth and its mechanism in Caenorhabditis Elegans; investigate radiation protective effect of resveratrol and its mechanism in Caenorhabditis elegans.Methods: (1) Wild-type C. elegans at L1 stage were irradiated by 60Coγ-irradiation with 50Gy, 100Gy and 200Gy respectively, and detected the lifespan, locomotion, reproduction and the memory of irradiated C. elegans. (2) Wild-type C. elegans treated with the different concentration (10μM,100μM,1000μM) of resveratrol, the lifespan of C. elegans and its experimental intervention concentration were detected effective. (3) With the effective concentration of resveratrol on wild-type C. elegans, the lifespan, locomotion, reproduction and the memory of irradiated C. elegans were detected, and assessed the protective effect of resveratrol on the radiation. (4) The level of oxidative stress were detected in C. elegans with CF1553 [muIs84 [pAD76 (sod-3::GFP)]] and DCF. (5) The mtDNA copy number of C. elegans was determined with the quantitative PCR. (6) The changes of lifespan were observed with VC199 [sir-2.1(ok434)], to investigate its some activity mechanism of the protective effect of resveratrol in Caenorhabditis elegans on radiation.Result: (1) Life span of irradiated wild-type C. elegans were shortened along with the increased radiation dose; the locomotion suffered injury obviously and memory deficits were caused with 60Coγ-irradiation exposure; the amount of offspring was obvious reduced along with the increased radiation dose, and when the dosage increased to 200Gy, all maternal worms became sterile. (2)Dietary resveratrol with the different density extended life span of wild-type C. elegans. As there was no significant difference between 100μM and the higher density, all other experiments were performed with 100μM resveratrol. (3) The lifespan was extended, the locomotion was enhanced, the memory was improved, laying-egg was increased, and the egg survival percentage was increased with resvertrol pre-treatment. (4) The oxidative stress was significantly attenuated with resvertrol pre-treatment in 60Coγ-irradiated C. elegans. (5) The mtDNA copy number was increased with resvertrol pre-treatment in 60Coγ-irradiated C. elegans. (6) Resveratrol pre-treatment also extended life span 60Coγ-irradiated sir-2.1 mutants.Conclusion: (1) Caenorhabditis elegans is a useful model of animal on irradiation-related damage, which can be examined the damage of growth caused by 60Coγ-irradiation. (2) Resveratrol has the good protective function to the 60Coγ-irradiated, such as extended the lifespan of wild-type C. elegans, enhanced its locomotion, improved the memory and protected the reproductive system. (3) The protective effect of resveratrol on radiation is the strong ability to protect from oxidative stress and protective effects to mitochondria. (4) The sir-2 independent mechanisms play an important role in the protective action of resveratrol.