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Study On Biology And Influence Of Methyl Eugenol On Biology Of Oriental Fruit Fly’s Genetic Sexing Strain

Posted on:2011-06-01Degree:DoctorType:Dissertation
Country:ChinaCandidate:Q L GuoFull Text:PDF
GTID:1223330467952810Subject:Biological control
Abstract/Summary:PDF Full Text Request
In order to further optimize the mass rearing technique of genetic sexing strain(GSS) of oriental fruit fly, and understand the influence of methyl eugenol on it, the biology of genetic sexing strain and the influence of ME on it were studied. The results showed that:1. Biology of males from GSS8days after emergence males reached sexual maturity. There was no significant difference in the continuous mating ability between males from GSS and wild males.46.67%of females would mate again. The average egg hatch rate of multi-mating females was higher than that of mating only once, and the eggs of no-mating females did not hatch. The average egg hatch rate of continuous collected was lower than that of interval collected. Egg hatch rate would decline with the females age increased and there had no significant difference among11-25day age of females. When the density increased from1000per cage gradually to2200per cage, the single female fecundity increased first and then gradually decreased. The relationship between single female fecundity (Y) and population density (X) was simulated by the equation Y=-0.0002X2+0.6851X-60.792(R2=0.9013>R0.05). From the equation, the theoretical maximum525.91of single female fecundity could be obtained when the density was1712.75per cage, and the average single adult shared15.76cm3cage space, occupied an area of3.15cm2of the cage wall. The mortality of a generation was47.19%in artificial conditions and that was26.58%in larva stage. The relationship between pupaes mortality(Y) and submerging time(X) of mature larvae was simulated by the equation lgY=1.5148lgX-1.0092. The ratio of white and brown pupae of offspring was1:1.39, and the relationship between white pupae reverse mutation rate and generations was simulated by the equation Yl=0.4941X-2.5478(Y:reverse mutation rate, X:generation).In the same time, the equation of the relationship between brown pupae reverse mutation rate and generations was obtained,ie.Y2=0.5804X-1.793(Y:reverse mutation rate, X:generation). The relationship between sex ratio (X) and effective eggs of single male (Y) could be gotten by the equation Y=0.747X3-36.408X2+887.368X-102.805in a certainn density. From the equation it would be optimal for mass reasring GSS when the sex ratio((?):(?)) changed from1.5:1to2:1,. Biology of sterile males from GSSEmergence rate, ME tropism and longevity of males could be reduced significantly by radiation, but the flight ability was not significantly affected. The relationship between mortality (Z1) and age (N1) of sterile males was simulated by the equation lg(Z1)=-20.679/N1+0.4805.2The influence of MEon biology of males from GSS7days after emergece males would show the trend to ME. Re-feeding rate of males emerged25d was not significantly affected by the time (1-24h) of feeding ME. With males feeding ME for2h, there was no significant difference in re-feeding rate afterl5,25,35d. The mating success and mating competitiveness would decrease with the increasing of males age. There was no significant influence on mating success rate of males feeding ME at different age,but the mating competitiveness of male could be improve significantly by feeding ME.The proportion of males with non-trend to ME would increased with generations of slecting.however, the proportion would stabilize about30%.Results also showed that males trending to ME were significantly lower than control males since F2generation, that had equal mating competitiveness to wild males.The influence of ME on bioloy of sterile males from GSSFeeding ME could substantially increase the mating competitiveness of sterile males, but would not increase both the total number of multiply mating and the continuous mating frequency of sterile males. At the same time, sterile males fed methyl eugenol did not significantly affect the remating of females at5days after the initial mating, but did increase the remating frequency of females at10and15-days after the initial mating. If both sterile males and wild males were exposed to ME together the sterile males had higher mating competitiveness than wild males.
Keywords/Search Tags:Bactrocera dorsalis (Hendel), genetic sexing strain, methyl eugenol, males, sterile males
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