The Study On Process Of Extraction With Huanglianjiedu Decoction And Separation Mechanism Of Macroporous Resin Used Refining The Extraction With Chinese Medicine Compound

Chinese Medicine Compound, the quintessence of the theory of Traditional Chinese Medicine (TCM) and the main form of clinical application and foundation of proprietary Chinese Medicine production is extensively extracted with water and the decoction is applied to treat with many diseases. Thus, the substances that we separated form the decoction of TCM and Chinese Medicine Compound is helpful for our study up to now, and generally this substance is safe and effective to be used in clinic. How to maintain its advantage so as to Chinese Medicine Compound can play a better and more convenient role in modem society is important. In order to fully demonstrate the safety and effectiveness of Chinese medicine’s preventing and have been treated diseases effectively for several thousand years, we must study on "inheritance" and "innovation" with Chinese Medicine Compound. Up to now, the basic research on the decoction of Chinese Medicine Compound and its refining process is essential, because Chinese Medicine Compound has more constituents than a single herbal medicine, and the study is so difficult that we use modem technology.This dissertation is one of the studies on a complex system of Chinese Medicine Compound refined by the means of industrial isolation. Huanglianjiedu decoction is selected as experimental research system. Firstly, we took the study on the extraction process, and then, physical pharmacy were applied to investigate the active of the fractions that separated from the decoction, and statistical method was used to establish a forecast mathematical model so as to guide the extracting with Chinese herbal medicine. At last, we used macroporous resin technology that has been considered as modern separation and purification technology and widely applied in industrial production to separate and purify the Huanglianjiedu decoction. In this process, the data mining and knowledge discovery in database were applied to elucidate the separation and purification mechanism of macroporous resin. All of these studies are aimed to establish scientific expressional reference model when using this technology in modem medicine production, and ensure the safety and stability of Chinese Medicine Compound after being refined.The premise study on separation and purification with Chinese Medicine Compound is the study on extraction with it. In this paper, firstly, we set our attention on precipitation that is obtained from decoction by extracted with water. Then, dynamic pseudomonas aeruginosa was used to study on the dissolution changes of multiple indexes substances from the extraction, and the changes of physical and chemical characterization parameters in this process were measured. In the end, mathematical and statistical methods were used to explore the mechanism of precipitation, and try to establish the relevant mathematical equations related guidance from Chinese herbal medicine.In this dissertation, we developed a novel idea of applying modem information technology to study the process of extraction and refinement in the field of Chinese drugs pharmaceutics.The process of separation and purification with TCM is the most crucial link in the production process of proprietary Chinese medicines, and the key issue which restrains the improvement of the Chinese herbal medicine quality. The technology of macroporous resin that play an role on adsorption_separation with TCM, as one of the high technologies that has been used in Chinese pharmaceutical industries must be promoted.In this dissertation, Chinese Medicine Compound, considered as a special whole group of constituents was separated by "group selection". On the ground of modern separation scientific theory, we set about the common key issues which currently constraint macroporous resin technology, we combined advanced laboratory technology such as physical and chemical sciences to study on isolation and nature from the start, and then initially established a scientific, objective technical system which represents the separation characterization of Chinese Herbal Extract and recognized the physical and chemical properties of solution system (viscosity, turbidity, conductivity, pH, etc.) and the main chemical composition characteristics (pectin, starch, Tannins and key indicators components) as the main component.In this dissertation, data mining methods were used to study and analysis competitive adsorption between different constituents groups in the refined process after the physics, chemistry, pharmacology and other data parameters were measured during the process of isolation with the decoction. This is the study on the characterization and kinetics of relevant components’ solute transportion and proliferation. Through data mining and knowledge discovery, we systematically obtained a large number of data that changed from diverse and complex to simple, that is, through this study, we can mechanically indicate the component’s movement in the macroporous resin, and then quantify the difference between multi component and the effective parts to achieve the target separation.In this dissertation, the main studies are as following:1. The method of HPLC was used to study the quality control of these four Chinese medicine herbs, which are Rhizoma Coptidis (Huanglian), Cortex Phellodendri (Huangbo), Radix Scutellariae (Huangqin) and Fructus Gardeniae (Zhizi) in this prescription. All kinds of herbs of different sources were collected for the study on the origin and content, so we could ensure the qualified and controlled original ingredients to be used in the experimental study.2. The Change laws with Huanglianjiedu decoction in dynamic extracting process was detected by the method of indexes composition and physicochemical feature parameters through extracting with water from 10min to 120rain. The methods of UV and HPLC were used to study the dissolution laws of the different the indexes constituents including alkaloids, flavonoids, berberine, jatrorrhizine, palmatine, baicalin, meanwhile, the changes on physical and chemical characterization including pH, conductivity, salinity, viscosity, turbidity of parameters system were detected. The quantity of solid was measured to estimate the rate of precipitation in dynamic process. Therefore, we can make a comparative analysis by combining the visual analysis and mathematical software analysis to detect the correlation between the the physical and chemical characterization parameters and components, and the correlation between characterization of the physical-chemical parameters and sedimentation rates, and the correlation between the composition and the rate of precipitation which we attempt to establish the relevant mathematical equations and models with, and explore the mechanism produced in this process. All of these provide basic evidence for the dynamic research in the process of Chinese Medicine Compound being boiled.3. The comparative study on adsorption kinetics of single ingredients with nine different macroporous resins through static adsorption method. The methods of HPLC and UV were used to measure the quantity of relevant indicator constituents whose constant of adsorption rate, adsorption capacity and elution volume of different macroporous resins were detected closely. Different constituents have different rate of adsorption and elution, thus, the adsorption kinetics curve which the different constituents were drew with a different macroporous resin. Those data were related to the comparative study on adsorption kinetics in the process of adsorption.4. According to study 1, those data, such as the constant of adsorption rate, the adsorption capacity and the elution volume of alkaloids, flavonoids were measured by the methods of HPLC and UV, and the different adsorption kinetics curves were drew, meanwhile, the single constituents, such as berberine, jatrorrhizine, palmatine, baicalin were studied with the same method. Then we studied on kinetics changes in the process of compound adsorption, and compared the changes with the related indicators of single ingredients. Meanwhile, one kind of resin AB-8 was chosen to observe the adsorption force under two different temperatures.5. Beginning with the objective nature of the complex system deriving from Chinese medicine herbs’ water extraction, the physical-chemical methods were used to study on the physical and chemical parameters of liquid system in the process of single ingredients and compound adsorption. In this study, static and dynamic adsorption methods were also chosen to determine pH, conductivity, salinity, viscosity, turbidity changes of those liquid samples in every steps, e. g., adsorption, water solution in the dynamic process, and elution. Thus, we detect deeply the change laws of these characteristic physical and chemical parameters to analysis the reasons for their changes.6. The comparative study on adsorption kinetics of single ingredients with nine different macroporous resins through dynamic adsorption method. The methods of HPLC and UV were used to measure the quantity of relevant constituents including alkaloids, flavonoids, berberine, jatrorrhizine, palmatine, baicalin and geniposide. Thus, we calculate the transfer rates and compare their quantities of solid between differentsamples.7. We used inhibiting of platelet aggregation tests in vitro to compare the separation and purification products from nine different macroporous resins, and compared pharmacodynamics indicators between the products and samples before being refined.8. Through data mining software, we set about study on data mining that be settled to detect the correlations among "characteristic parameters of macroporous resin adsorption-the amount of physical and chemical parameters before and after Chinese Medicine Compound being extracted and refined-the indicator components feature before and after Chinese medicine compound being extracted and refined-the characteristic parameters ofmacroporous resin adsorption kinietics-pharmacodynamica indicator characteristic parameters". Thus, we can fit different curves to build the relevant mathematical equations, and to detect mechanism of the separation and purification of the water extract of Chinese Medicine Compound by the means of macroporous resin. Then we look for the law to provide ideas and evidences which aim at designing a kind of special resins used in the complex system of Chinese Medicine Compound.9. On the base of data mining and knowledge discovery, we designed five different resin compositions so as to begin with static and dynamic adsorption experiments. The amount of relevant indicator components, such as alkaloids, flavonoids, berberine, jatrorrhizine, palmatine, baicalin and geniposide were measured, and these data were used to take a comparative study on the relevant adsorption parameters and curves which provide evidences for the data mining and the related knowledge discovery.10. On the point of micro-perspective, we took in the research methods of microscopic particles so as to set about a comparative study on changes of nine different macroporous resins with their microstructures during the process from adsorption to elution by means of canning electron microscopy.In this dissertation. The innovations are as following:Under the guidance of Traditional Chinese Medicine theory, by the means of collecting amount of parameters of Chemistry of Chinese Materia Medica, Physical Chemistry and adsorption kinetics, the mechanism of extracting and refining of Chinese Medicine Compound was explored from the micro and precise point.1. It’s the first time that we apply dynamic study on extraction progress with Chinese Medicine Compound: multi-indicators detecting at the same time, combining the changes of the physical and chemical characterization parameters in the system, mathematical and statistical methods being used for exploring the dissolution law of constituents of Chinese medicine compound boiled with water, which providing a new method of inspecting craft model for overall evaluating dissolution situation of active constituents extracted by water, and further establishing characteristic physical and chemical parameters for quality control of extraction process on line.2. Under the guidance of the theories of modern scientific isolation, On the base of data mining and knowledge discovery, we focus on the mechanisms of separation and purification with macroporous resin as using to refine Chinese herbal medicine. In this dissertation, it’s the first time that we raise a novel concept of "based on new knowledge discovery of refining with assembly macroporous resin", which offered a new refining technique of macroporous resin to complex system of Chinese medicine compound, and it’s an important step for obtaining effective substance from Chinese medicine compound.3. We take a comparative study on the changes of its own external and internal micro-perspective shapes before and after different macroporous resins being used to refine Chinese Medicine Compound by the means of electron microscopy scanning for particulate research. Thus, we can analysis some relevant properties between Chinese Medicine Compound and the nature of macroporous resin during the refining process.