It is of key importance to recognize, detect and measure seawater Ca2+, in-vivo trace elements, environmental heavy metals in the fields of biology, chemistry, medicine and environmental science. Fluorescent sensors are useful and powerful tools in the detection of metal ions, because of their simplicity, excellent selectivity, high sensitivity and found widespread applications in biological field, environmental monitoring, and many other fields. Based on the principle of Photo-induced Electron Transfer (PET), molecular recognition and fluorescence sensing, we designed and synthesized a series of reusable fluorescent materials for recognition, detection and measurement of Ca2+in seawater, trace elements in vivo (Zn2+), heavy metal cations (Cd2+, Hg2+) in the environment and in vivo. All the fluorescent sensors we developed were thoroughly studied on their molecular structures, selectivity towards metal ions, sensitivity and cell imaging. All the compounds obtained were characterized by1H-NMR,13C-NMR, MS, and their purities were evaluated by high performance liquid chromatography (HPLC) and reached above99%.1ã€We designed and synthesized a series of4-amino-1,8-naphthalimide-based fluorescent sensors and studied their selectivity and sensitivity. Compared to N1and N3, N2and N44-amino-1,8-naphthalimide-based fluorescent sensors show more excellent selectivity.2ã€We designed and synthesized a series of ionized calcium sensors and fully studied their UV-vis absorption spectra, fluorescence emission spectra.4-Amino-1,8-naphthalimide-based fluorescent sensor, with phenyliminodiethoxyacetic acid as receptor, can be immobilized on the aminocellulose. It has been used for continuous monitoring of the calcium ion concentration in seawater with high selectivity against magnesium ion.3ã€We developed a series of4-amino-1,8-naphthalimide-based Zn2+fluorescent sensors and comprehensively investigated their selectivity, sensitivity and imaging in living cells. The Zn2+fluorescent sensors have good membrane permeability and can be used as a sensor for detecting Zn2+in living cells.4ã€We designed and synthesized a series of mercury sensors and made heavy studies on their UV-vis absorption spectra, fluorescence emission spectra (selectivity, sensitivity) and imaging in living cells.4-Amino-1,8-naphthalimide-based fluorescent sensor can be immobilized on the aminocellulose and was used for continuous monitoring of the mercury ion concentration in the environment. The Rhodamine-based and fluorescein-based Hg2+fluorescent sensors have good membrane permeability and can be used as applicable sensors for detecting Hg2+in living cells.5ã€We developed a4-amino-1,8-naphthalimide-based Cd2+fluorescent sensor and comprehensively studied their selectivity, sensitivity and cell imaging in living cells. The Cd2+fluorescent sensor have excellent selectivity against other metal ions, high sensitivity towards Cd2+and good membrane permeability. It is applied successfully to detect and image Cd2+in living cells. Besides, it is high selectivity against Zn2+, which is better than all the reported Cd2+fluorescent sensors. It also showed the different fluorescence intensity in cell imaging between the normal cells NIH-3T3and the cancer cells PC-3. |