The Synthesis And Properties Of Fluorescence Chemical Sensor Based On Triphenylamine And Naphthalene As Chromophore Abstract For Zinc Ions

In the human body,zinc is the second rich transition metal element;zinc is involved in many biological processes in the human body,such as enzyme regulation,gene expression,structural cofactors,and neural signaling.Zinc dysregulation can lead to a variety of diseases,including Alzheimer's disease,prostate cancer,diabetes and epilepsy.In addition,excessive zinc in the environment may reduce soil microbial activity and is not conducive to the growth of crops.Therefore,the research on the detection of zinc ions has attracted more and more attention from researchers.Moreover,the fluorescence analysis method has the advantages of high selectivity,high sensitivity,versatility,relatively simple processing,and real-time detection.Therefore,it is very necessary to synthesize a highly selective and highly sensitive fluorescent chemical sensor.This article has carried out the following work:1.The concept of fluorescence,the mechanism of fluorescence generation,the mechanism of fluorescent chemical sensors,and the research progress of triphenylamine and naphthalene fluorescent chemical sensors are introduced.2.A fluorescent chemical sensor TPA-HPH was synthesized from triphenylamine derivatives and 2-hydroxybenzohydrazide.The UV and fluorescence response experiments of the sensors TPA-HPH and Zn²⁺led to the conclusion that in the solution of V?acetonitrile?:V?water?=6:4 with pH=7.36,the probes TPA-HPH and Zn²⁺were 1:1.The coordination ratio of is complexed,and the complexation constant is 6.42×10⁴M^-1.The fluorescence intensity of probe TPA-HPH has a good linear relationship with the concentration of Zn²⁺in the range of Zn²⁺concentration from 0to 10?M?R2=0.9915?.The detection limit is 7.3×10^-10 M;in addition,other metal ions do not interfere with the fluorescence measurement of Zn²⁺by the probe TPA-HPH,but the anion EDTA can reverse the fluorescence of the TPA-HPH-Zn²⁺system.The coordination mechanism of probe TPA-HPH and Zn²⁺was investigated by hydrogen spectroscopy and infrared spectroscopy.The fluorescence enhancement mechanism of probe TPA-HPH in response to Zn²⁺was proposed based on PET mechanism and carbon and nitrogen isomerization.Fluorescence imaging using the probe TPA-HPH to HeLa cells has achieved good results.3.A fluorescent probe NPE-HPH was synthesized from 2-hydroxy-1naphthaldehyde and 2-hydroxybenzoyl hydrazide.The UV and fluorescence response experiments of the probes NPE-HPH and Zn²⁺led to the following conclusion:in the solution of V?methanol?:V?water?=1:9with pH=7.36,the probes NPE-HPH and Zn²⁺were 1 The coordination ratio of 1:1 was complexed,and the complexation constant was3.42×10⁴M^-1.The fluorescence intensity of probe NPE-HPH showed a good linear relationship with the concentration of Zn²⁺in the range of Zn2+concentration from 1 to 9?M?R2=0.99?.The detection limit is0.0456?mol/L;in addition,other metal ions do not interfere with the fluorescence measurement of Zn²⁺by the probe NPE-HPH,but the anion EDTA can reverse the fluorescence of the NPE-HPH-Zn²⁺system.The coordination mechanism of probe NPE-HPH and Zn²⁺was investigated by hydrogen spectroscopy and infrared spectroscopy.The fluorescence enhancement mechanism of probe NPE-HPH in response to Zn²⁺was proposed based on PET mechanism and carbon and nitrogen isomerization.Fluorescence imaging using the probe NPE-HPH to HeLa cells has achieved good results.