Classfication And Taxonomy Research On The Nematodes Of Longidorids And Tripylids In Shanxi Province, China

The morphology and rDNA molecular characterazition for Longidorids and Tripylids in Shanxi province were studied in the paper. The results showed that 12 species nematodes in five genera were identified, which including four new species, four new records in China, two new records in Shanxi province and a male report. And the phylogenetic relationships among five genera were analyzed using data from the near full length small subunit (SSU) and D2/D3 segments of LSU of the ribosomal rRNA genes.In addition, the nematodes biodiversity around the roots of diseased Pinus bungeana Zucc in Fenyang city was analysed and the relationship between Longidorids population quantity and disease degree were discussed in the paper. The results are as following:1. Longidorus viburnum sp. nov. was collected from the soil around the roots of Viburnum dilatatum Thunb at Jincheng city, Shanxi province, China. The new species is characterized by having a median body length (L=4.85 mm), odontostyle length 103μm, lip region width 22μm, lip region flattened and continuous, amphid pouch elongate, funnel-shaped, not lobed, guide ring at the posterior of ondotostyle and 59 μm from anterior end of body, tail bluntly conoid, males with conspicuous spicule and 8 or 9 supplements near the anus. The code for identifying the new speics using the polytomous key of Chen et al (1997) is:A4-B4-C5-D3-E4-F2-G1-H1-I2.2. Three known species of the genus of Longidours Micoletzky 1922 are re-described in the paper. Longidorus bernardi Robbins & Brown 1996 and Longidorus cedari (Khan, Saha & Seshadri 1978) Decraemer & Coomans 2007 are two records in China. And the male of Longidorus jonesi Siddqi 1962 is reported firstly in the paper.3. The paper describes two known species, a new record in Shanxi province Xiphinema brevicolle Lordello & Da Costa 1961 and Xiphinema insigne Loos 1949 in the genus Xiphinema Cobb 1913. Morphometrics of first, second and third juvenile stages of Xiphinema brevicolle are presented in the paper.4. The paper describes a new species and gives a new record in Shanxi province of Tripylina Brzeski 1963. Tripylina puxianensis sp. nov. is characterized by having a robust body, six long and four short cephalic setae in a single whorl, dorsal tooth conspicuous, triangular, pointing towards to ventral side, two subventral teeth similar to dorsal tooth, triangular, pointing towards to dorsal side, one ventromedian seta in cervical region, female genital system mono-prodelphic with no post-vulval sac and a pair of subdorsal caudal setae on anterior part of tail. For the first time, Tripylina zhejiangensis Pham, Wang, Zhao & Zheng 2013 is described in Shanxi province, China.5. The paper discribes a new speices and gives a new record and a re-descripiton of a known species of Trischistoma Cobb 1913. Trischistoma taiguensis sp. nov. is characterized by having a slender body, six longer outer labial setae and four shorter cephalic setae separated in two circles, cardia between pharynx and intestine inconspicuous, gonad prodelphic and post-vulval uterine sac absent and a pair of caudal setae on the tail. For the first time, Trischistoma pellucidum Cobb 1913 is recorded from Shanxi Province, China.6. This paper describes a new species and a known species of the genus Tripyla Bastian 1865 from Shanxi province, China. Tripyla wutai sp. nov. is characterized by having a long body length (L= 1447-1644 μm), six outer labial sensillae and four short cephalic setae arranged in two separate whorls, outer labial sensillae outward directed, not reaching beyond lips, a wedged dorsal tooth posterior to subventral teeth, cardia between pharynx and intestine conspicuous, female reproductive system didelphic, relatively anterior vulva (V=47-56%), widest part of the tail at the anus and short spinneret (2-4 μm). Tripyla setifera had been reported from China but without a detailed description which is provided in the paper.7. In addition, the phylogenetic relationships among five genera were analyzed using data from the near full length small subunit (SSU) and D2/D3 segments of LSU of the ribosomal rRNA genes. And diagnostic keys for identification of Longidorids and Tripylids occurred in China are given in the paper.8. The nematodes biodiversity around the roots of diseased Pinus bungeana Zucc in Fenyang city was analysed, and the results showed that the needle nematode often occurred in the 20～40 cm deep of the roots, and it has more quantity needle nematode around the more severe diseased tree roots. So it has some relationship between the quantity of needle nematodes around the roots of Pinus bungeana Zucc and the disease degree.