Study On The Community Of Arbuscular Mycorrhizal Fungi And Host Plant And Their Ecological Restoration Function For The Suface Mine Spoil Bank Under Different Revegetation Management

Coal resources are richer in our country western semi-arid, arid region. Production scale of Open pit mine is huge. Environmental pressures of is more and more big. Task of mining vegetation restoration and ecological construction is arduous. HeiDaiGou opencast coal mine is the larger modernization coal production enterprises. Vegetation status are evaluated popularly and identified as monitoring indicators in the domestic. Study on ecological restoration function of reclamation plant and combination to vegetation succession, diversity of soil biological recovery, soil structure and function is not profound enough. Spoil bank of reclamation seven and fourteen years is as object of study. The physicochemical properties of soil, AMF and host plant of spoil bank are systematically studied. Soil and plant samples are collected by collaborative sampling method in the same plots. Considering the rDNA polymorphism of AM fungi and the economic feasibility, AM fungi are isolated and identified spore from soil sample by the morphology method. The relationship between soil factors, AM fungi and host plant are studied by the Canonical Correspondence Analysis and Detrended Canonical Correspondence Analysis. Restriction factors of the dump recovery of microorganism and plant community are explored. By controlling the experimental, function of AM fungi and host plant improving soil restrictive factors is studied. The main conclusions are as follows.1. Eighteen AMF species are isolated and identified.18 species belong to 5 families and 9 genera.80 plants belonging to 10 families and 9 genera are identified.Through the method of TWINSPAN,8 plant communities and 11 AMF communities are divided. The results of TWINSPAN are consistent with DCA.The results of plant community succession with over times under different reclamation show that the stability and species diversity of plant community generating from reclamation mode of Pinus tabulaeformis and Agropyron mongolicum is better than from reclamation mode of Populus alba var. pyramidalis and Medicago sativa..Sequence of species succession is in order that Artemisia ordosic、Setaira viridis、Salsola collina、 Agriophyllum squarrosum�'Artemisia ordosic、Leymus secalins、Cleistogenes squarrosa、Poa sphondylodes (7a)�'Artemisia ordosic、Calamagrostis epigeios、Stipa beviflora、Caragana davazamcii (14a).Artemisia ordosic、Calamagrostis epigeios、Caragana davazamcii is dominant species among the natural recovery species. These species as native plants are recommended to vegetation restoration.The two data matrix of sample-plant and sample-environment are obtained by filtering plant of the important value less than 0.05. The results of Detrended Correspondence Analysis show that soil organic matter, soil moisture, soil fertility, reclamation mode, reclamation year were closely related with the first axis of ordination diagram of DCCA. These soil factors are positive effect on community species diversity. Soil bulk densities, content of clay are related with the second axis of DCCA ordination diagram and are negative effect on community species diversity of plant community. With the increase of organic matter, Community is in turn Ⅰ、 Ⅱ、Ⅳ、Ⅲ、Ⅴ、Ⅶ. With the increase of soil bulk density, Community is in turn Ⅷ、Ⅶ、Ⅵ、Ⅳ、Ⅴ、Ⅲ、 Ⅱ、Ⅰ.2. AM fungi species area is greater than the species area. AM fungal species area curve results show that sampling area is in accordance with AMF when the community relations between AMF and host plant are studied in spoil bank. In the process of vegetation restoration of spoil bank, Glomus deserticola, Glomus versiforme, Glomus intraradices, Claroideoglomus etunicatum, Diversispora spurca, Funneliformis mossea, Septoglomus constrictum, Rhizophagus diaphanum, Acaulospora rehmii, Glomus convolutum, Glomus versiforme are main constructive species and Acaulospora.spl is pioneer AMF. The distribution of AM fungal community is driven by the host plant community. DCCA method analyzes the two data matrix of Sample-AMF and Sample-soil environment. Soil moisture, soil bulk density are main factor of influencing AMF recovery. With the increase of soil moisture, AMF community is in turn ①,②,④,③,⑦,⑤,⑩,⑥,⑨ 8. Soil organic matter, soil moisture, soil fertility, reclamation mode, reclamation year are positive effect on community AMF recovery. With the increase of soil bulk density, AMF community is in turn ⑧,⑨,⑩,11,⑦,⑤,⑥,④,③,②,①. Soil bulk densities, clay content are negative effect on AMF community recovery.3.Inoculation experiments confirmed Mycorrhizal system of AMF-Caragana davazamcii and AMF-Calamagrostis epigeios could grow in the unreclaimed dump environment, effectively reduce the soil bulk density, increased soil organic matter and soil soluble N, P, K content, significantly improve the soil physical and chemical properties. Mixed AM fungi inoculation effect on Root Traits of plants than single inoculation is obviously. Mixed inoculation significantly increase the root area, root volume, number of root tips of Caragana davazamcii、Calamagrostis epigeios. Inoculation treatment significantly increase the plant height, biomass of Caragana davazamcii、Calamagrostis epigeios and have no significant effect on the number of leaves.Mycorrhizal system of mixed AM fungi inoculation outperform other inoculation treatment on soil improvement effect, especially mycorrhizal system of Funneliformis mosseae, Glomus versiforme, Glomus deserticola inoculating Caragana davazamcii. They can be priority application in mine land reclamation in the future. But there have certain difference between pot experiment and situ regional experiment. The results of pot experiment need to be further verified by situ regional experiment, and then the results can be applied.For the future will be a dump, the dumping technology of leading to high soil bulk density does not use. For the existing no reclamation dump, the small agricultural machinery is taken to loose topsoil. Reclamation measures of increasing the content of sandy topsoil of 30 cm, reducing the content of clay, improving the soil texture and vegetation restoration applying mixed AMF inoculation Caragana davazamcii are taken.For dump of reclamation but poor effect, plant of strong stress resistance is grown. Mycorrhizal interventions help plants better adapt to the bad soil environment, promote the evolution of plant community as soon as possible. The ecological environment restoration and improvement of spoil bank become a reality as early as possible.