Study On The Microchip Capillary Electrophoresis System And Its Applications On Clinical Diagnosis

Microchip Capillary Electrophoresis (MCE) system is a newly rapidly developed research technology and has extentive application perspective. Based on the routine capillary electrophoresis (CE) principle, the microchannel network on the microchip is manufactured by the technology of Micro Electro Mechanical System (MEMS) on the substrate of glass, fused silica, polymers etc. Due to the advantages of small dimension and high thermal conductivity, MCE system can realize more rapid analysis. Though great development of MCE technology, there are still many problems to be solved, such as standard micromachining of microchips and rate of finished products, optimization of injection methods. The application of clinical diagnosis of MCE system begin to be attached importance to, however, which is just at the beginning.In this dissertation, for solving the problems above and clinical diagnosis application of MCE system, after the principle of MCE system is discussed, according to the characterization of MCE, the optimization of injection methods was performed, the stardand micromachining of CE microchips was solved. A low-temperature bonding method was set up, which improved the rate of finished products greatly. What's more, the applications of clinical urinary proteins and LDH serum in clinical diagonosis were studied.The topics of this dissertation are presented below:1. The capillary electrophoresis principle and the recent developments of MCE system were reviewed. On the base of it, a research scheme was brought forward.2. Due to the great impact of MCE injection technology on separation performance, a double-focusing injection method was studied with numerical simulation approach, and the experimental setup was builded up to test the simulated results. The results proved that this method could improve the shape of delivered sample plug to realize a well-defined sample plug into the separation microchannel, which could improve the resolution and separation performation.3. Due to the low rate of finished products on traditional bonding methods, a low-temperature bonding method for microfabrication of quartz microchips has been developed. The bonding procedures were not sensitive to surrounding and could be performed in a routine lab. The bonding strength was 5.66 MPa and 95% rate of finished products. For reducing the cost of microchips, the based on PDMS microchip micromachining technology was also studied.4. According to the characterization of MCE and application of clinical diagonisis, a microchip-based uriary protein detection system was set up and employed to perform the analysis of clinical urinary protein from special patients.5. The microchips have rapid thermal conductivity and are easy to be integrated. Accroding to the principle of Peltier, a temperature control system for on-chip enzyme reaction is set up. A xenon-lamp-induced fluorescence detection system was set up and employed to perform the analysis of LDH serum. The results were accordant to the one of routine method. However, the analysis time in MCE system was shorter. The whole experimental process could be accomplished in 10min.The study of this dissertation showed that MCE system has some advantages such as high speed and sensitivity, which have extensive application perspective.