Studies On The Eelectrochemical Immunosensor And Its Primary Applications On Veterinary Medicine

We have designed and fabricated a kind of electrochemical immunosensor by using the theory of immunology and the technology of electrochemistry and apply the sensor to detect reactions of antibody and antigen on veterinary. By this way, we surpose to set up a new quick, sensitive, specific detecting method of antibody - antigen reactions for the veterinary practice use.The contents of the research mainly include three aspects:Firstly, it is to select proper materials for designing and fabricating the electrochemical immunosensor. Secondly, using the proper method to set up the experiment conditions and judge the characters of the sensor. Thirdly for examining the result of the electrochemical immunosensor made, we use the sensor to detect antibody-antigen reactions of several common diseases of parasites and microbes.Working electrodes, auxiliary electrodes, referent electrodes, reaction pools and so on are all the important components for making the electrochemical immunosensor. It is very important to select proper materials to design and make these components.Through a series of selecting experiments, we found the proper matrix materials and utilized these materials to make the electrodes and reaction pools with good properties. Finally, we successfully made a system of electrochemical immunosensor by using these compenonts.It is the key technology to immobilize biomolecules such as antibody and antigen onto the matrix of electrodes and maintain their reactivity and stability. We use the methods of chemical modifications to create active basal molecules on the matrix surface of electrodes and successfully immobilize the biomolecules onto the matrix. The experiment results indicated the immobilized antigen still keep its good reactivity and stability and can use for detecting reactions of antibody-antigen.The key procedure for practice use of the sensor is to set up its experiment conditions. We sut up the experiment conditions by using the mature and simple voltammetry.The results of detecting the properties of our sensor indicated the sensor has good stability, selectivity, adaptability and the specific detecting effects. But as some conditions are confined the detecting RSD of the sensor is still not so good. So the sensor is not suit to quantitative analysis at present.Our final aim is to use the sensor to detect reactions of antibody-antigen in veterinary clinic and field. So it is very important to exam their applicability in veterinary. In this study we had used the sensor to detect antibody-antigen reactions of Fasciolsis,ND, IBD and EDS. It is proved the sensor is fitted to use in detecting antibody-antigen reactions of these diseases. The results of detecting antibody-antigen reactions of Fasciola gegatica, ND, IBD and EDS by using the sensor basically conform to that with standard samples. Through mis experiment we think to set up a more reasonable standard for judgingthe clinic and field samples we still want to make further experiments.Through the primary studies, our sensor has showed many excellent characters such as adaptability and specificity, quick detection, simple operation ,small amount consumption of sample serums, miniature of the sensor, convenience to carry and the detecting instrument is cheap and so on. With the many excellent and special characters the sensor has displayed its light apply perspectives. We believe through further designing and experiments it is possible to use the sensor to quick detect reactions of antibody-antigen and diagnose diseases in veterinary clinic and fields.