A Study Of Species Identification For Forensic Ichthyology

Objective Forensic ichthyology can provide scientific evidence for criminal investigation and judgment. It is important to scientific management and conservation for fishery resources. The purpose of our study is to develop a new approach of identification species for forensic ichthyology.Methods A total of five rare and endangered species of Cypriniformes were selected as target fishes. They were Myxocyprinus asicvticus, Schizothorax davidi, Procypric rahaudi, Sinilabeo rendahli, Varicorhinus angastistomatus. Other twenty species of fishes were selected as control group. All fishes were collected from Yangtze River, Jialing River and Ya River as well as Fishery Markets in Chengdu and in Chongqing. Using primer design software, we designed universal primers that were employed to amplify the DNA fragment containing the entire nuclear ribosomal DNA internal transcibed spacer II (ITS2) region plus portions of the flanking 5.8S and 28S ribosomal RNA genes for Cypriniformes. Fish DNA samples were amplified by PCR with universal primers. The PCR products were analyzed by polyacrylamide gel electrophoresis with sliver staining. Recovered...