P38 MAPK In The Pathogenesis Of Allergic Rhinitis Rat Model

Partâ… Expression of p38 MAPK in nasal mucosa in allergic rhinitis ratObjective:To investigate the expression level of p38 MAPK protein and mRNA in nasal mucosa in allergic rhinitis rat model and controls.Methods:a model of allergic rhinitis was established in SD rats. The expression of p38 MAPK protein in nasal mucosa was detected by immunohistochemistry technique among allergic rhinitis rats and controls. The expression level of p38 MAPK mRNA in nasal mucosa was detected by means of real time RT-PCR.Results:The results show that the expression of p38 MAPK protein in nasal mucosa was significantly higher in allergic rhinitis rats than that in control rats (P<0.05). p38 MAPK mRNA expression in nasal mucosa was significantly higher in allergic rhinitis rats than that in control rats (P<0.05).Conclusion:The expression of p38 MAPK protein and mRNA was obviously increased in allergic rhinitis rat models, it indicates that p38 MAPK may involved in the pathogenesis of allergic rhinitis. Part IIExpression of p38 MAPK in PBMCs in allergic rhinitis rat and role on Th2 cytokines releaseObjective:To investigate the expression level of p38 MAPK protein and mRNA in PBMCs in allergic rhinitis rats, at the same time investigate the effect of p38 MAPK inhibitor SB 239063 on Th2 cytokines IL-4 and IL-5 production in PBMCs in allergic rhinitis.Method:A model of allergic rhinitis was established in SD rats. Blood samples were collected by cardiac puncture. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples by Ficoll-density gradient centrifugation. The expression level of p38 MAPK mRNA in PBMCs was detected by means of real time quantitative RT-PCR. PBMCs were cultured with or without various concentrations of p38 MAPK inhibitor SB 239063, and then IL-4, IL-5 levels of the supernatant were determined using sandwich ELISA.Result:The results show that mRNA and total p38 MAPK expression of AR rats was significantly upregulated compared with control rats (P<0.05). Phospho-p38 MAPK levels, which represent p38 MAPK activity, were greater in the AR rats relative to control rats. The ratios of phospho-p38 MAPK to total p38 MAPK levels were significantly greater in AR rats than that in control rats (P<0.05). p38 MAPK inhibitor SB 239063 inhibits IL-4, IL-5 production in a dose-dependent manner.Conclusion:It is concluded that p38 MAPK plays an important role in the regulation of Th2 cytokines release in allergic rhinitis rats. These suggest inhabit p38 MAPK pathway can reduce Th2 cytokines production and may rebound Thl/Th2 balance in allergic rhinitis.