Effect Of Restraint Stress On The Metabolism And The Expressions Of PPARs In The Rats And Intervertion By Pioglitazone

Objective:1.To investigate the changes of blood glucose and lipid of rats under restraint stress.2.To detct the expressions of PPARs in the liver,muscle,myocardium and fat in the rats under restraint stress.3,To explore the effects of pioglitazone on the metabolism of rats under restraint stress and its mechanism。Methods:1.Male Wistar rats were divided into four groups:control and rats under 1 week (R1),2 weeks (R2) or 4 weeks (R4) restraint stress. Blood glucose,TG,TC,HDL-C,LDL-C,FFA,corticosterone,noradrenaline and insulin were detected。The gene and protein expressions of PPARs,GLUT-4,LPL and CPT in the liver,muscle,myocardium and fat were evaluated by RT-PCR and western blot.2.Male Wistar rats were divided into four groups:control,rats under restraint stress,rats given both restraint stress and 10mg/kg-d pioglitazone (P10) and rats given both restraint stress and 20mg/kg-d pioglitazone (P20). Blood glucose,TG,TC,HDL-C,LDL-C,FFA,corticosterone,noradrenaline and insulin were detected. The gene and protein expressions of PPARγ,GLUT-4 and LPL in the fat were evaluated by RT-PCR and western blot. Probability values of 0.05 was considered as significant.Results:1.Compared with C1 group rats, blood glucose,TG,HDL-C,FFA,corticosterone,noradrenaline and insulin in the rats of R1,R2 and R4 group increased significantly, IAI decreased, LDL-C in R1 group also decreased, TC has no significant change.2.Compared with R group rats, blood glucose in the rats of P20 group decreased significantly, blood TG,FFA and insulin in the rats of P10 and P20 group decreased, IAI increased, blood TC,LDL-C,HDL-C,corticosterone and noradrenaline have no significant change.Compared with C2 group rats, blood HDL-C in the rats of P20 group increased significantly, blood HDL-C,corticosterone and noradrenaline in the rats of P10 and P20 group all increased, LDL-C decreased, blood glucose,TG,TC,FFA, insulin and IAI have no significant change.3.The expressions of PPARα,L-CPT mRNA and protein in the liver of rats of R1,R2 and R4 group were all significantly higher than that of control group, PPARβ/δ,PPARγ,GLUT-4 mRNA and protein were all significantly lower, the expressions of LPL mRNA and protein have no signigfican change.4.The expressions of PPARα,PPARβ/δ,PPARγ,GLUT-4,LPL,M-CPT mRNA and protein in the muscle of rats of R1,R2 and R4 group were all significantly lowered than that of control group.5.The expressions of PPARα,LPL,M-CPT mRNA and protein in the myocardium of rats of R1,R2 and R4 group were significantly higher than that of control group, PPARγ,GLUT-4 mRNA and protein were significantly lower, the expressions of PPARβ/δmRNA and protein have no signigfican change.6.The expressions of PPARα,PPARβ/δ,PPARγ,GLUT-4,LPL,M-CPT mRNA and protein in the fat of rats of R1,R2 and R4 group were all significantly lower than that of control group.7.Compared with R group rats, the expressions of PPARγ,GLUT-4,LPL mRNA and protein in the fat of rats of P10 and P20 group decreased.Compared with C2 group rats, the expressions of LPL mRNA and PPARγ,GLUT-4,LPL protein in the fat of rats of P10 group still decreased, the expression of LPLmRNA,PPARγ,GLUT-4,LPL protein in P10 group and LPL mRNA,PPARγ,GLUT-4,LPL protein in P20 group have no signigfican change。Conclusion:1.Rats under restraint stress have metabolic abnormality, with higer blood glucose and TG.2.The gene and protein expressions of PPARs in the liver,muscle,myocardium and fat were changed in the rats under restraint stress, which may have relationship with metabolic abnormality.3.Rats under restraint stress by pioglitazone treatment can inhibit metabolic abnormality by increasing the the expressions of PPARγ,GLUT-4,LPLmRNA and protein in the fat of rats.