The Study On The Anti-influenza Virus Mechanism Of The Tou Xie Jie Du Method

Based on the analysis of the influenza virus pathogenesis,the article uses the animal models of influenza and the models of cell and evaluates the lung index and mortality to indentify the effects of Jin Chai anti-vital capsule of animal models under differently immune status.Through the studies of the interaction of virus-cell and virus-body-immunity system and the uses of the methods of laser scanning confocal microscopy real-time fluorescent RT-PCR and Western-Blot,this experiment reveals the mechanism of Jin Chai antivital drugs on the molecular level and the effiency of the Tou Xie Jie Du to treat the influenza and provides the scientific proofs.The article consists of three parts:The first part reveals the machanism of influenza virus pathogenesis and the effects of traditional chinese medicine.The second part reveals the theoretical basis and the advantages of Tou Xie Jie Du.The third part reveals the machanism from two facets:virus-cell and virus-body-immunity system.1. Theoretical DiscussionThe influenza belongs to the Chinese medical syndrome of Shi Xing Gan Mao,Shi Sing Shang San and Shi Xing Yi Bing Because of the influences of the social development and environmental change and the effects of the people's habits and medical practices, the nature of pathogenic factors and the mechanism of influenza pathology no longer adhere to inherent rules.The explosion of the character of pathogenesis and the regulation of syndrome is an important task of treaditional Chinese medicine. 2 Experimental Study2.1 The role of prevention and treatment of influenzaUse the nose dropping method with influenza virus (FM/1/47 and PR/8/34 strains) to establish immunocompromised mouse model. Respectively do the theraputical administration and prophylactic administration to observe the lung weight and calculate the lung index and mortality.①Therapeutical effect:Jin Chai antivital capsules markedly decrease the lung index and mortality of mouse model with infuluenza virus and prolong the live time of the mouse model with infuluenza virus.③The antivital efficacy is not statistically significant between Jin Chai antivital capsules and Tamiflu.2.2 Mechanism of anti-influenza virus②Use Real-time PCR to observe the levels of HA-mRNA expression of influenza. The results show that Jin Chai antivital capsule can significantly reduce the levels of HA-mRNA expression of influenza and suggest the efficacy of Jin Chai antivital capsules through the intervention of the virus adhersion.②Use the combination of laser scanning confocal fluorescencent dye which is sensitive to PH value, to observe Virus-cell membrane fusion, The results show that Jin Chai antivital capsule can improve the levels of PH value, and suggest the efficacy of Jin Chai antivital capsules through the intervention of the Virus-cell membrane fusion.③Use Real-time PCR to observe the levels of PA-mRNA expression of influenza. The results show that Jin Chai antivital capsule can significantly reduce the levels of PA-mRNA expression of influenza and suggest Jin Chai antivital capsules effect on the Transcription and Replication.④Use the Elisa method of fluorescence to observe the Jin Chai capsules on the NA, the results show that Jin Chai anti-viral capsules do not inhibit the efficacy of NA activity.⑤Use Real-time PCR and Western-Blot method to observe the Jin Chai anti-virus Capsule effect on the interferon-y and natural anti-virus protein-interferon induces transmembrane protein,.the results show that Jin Chai anti-virus Capsule can improve the expression.of the interferon-yand interferon induced transmembrane protein expression.