Biological Basic Research, The Rat Model Of Hepatic Depression Syndrome Of Liver Qi Stagnation Law

Objective:To investigate the effect of CHSGS on the example rats which suffer Liver-Qi Stagnation Syndrome, plus with the study of CHSGS effect on Liver-Qi Stagnation Syndrome through the way of endocrine, immunization and signal transduction pathways, it reveals the way of relieving depression of the liver-qi and sets a basic study on relieving depression of the liver-qi.Method:1 The set up of liver-qi depression rats:we divide those rats into regular group and model group by adopting Open field test, forty each. It takes four weeks for them to infect liver-qi depression by keeping them alone and tools constraints-breeding. We observe those rats appearance weekly; detecting blood serum CORT, GAS, IL-2, blood plasma ACTH,MTL and weighing rats weight, spleen and thymus gland.2 Compared with the Chaihushugan San group:the rats are divided into regular groups, model group, CHSGS group and SJZT Group by adopting Openfield test, ten each. The model group will be bred two weeks by keeping them alone and tools constraints. The regular group and model group will be physiological saline lavaged once a day. CHSGS group and SJZT group would be lavaged lml/100g once a day. Two weeks later, we observed each group on behavioral biology including Open field test and Forced Swimming test after the two hours later after the last lavage. Then we observe the actions of rats, use RAImmune to detect blood serum CORT,GAS. IL-2, blood plasm MTL; weigh body mass of rat and weight of spleen and thymic.3 The Chemistry study of CHSGS group and liver-qi depression group ERK-CREB cell Signaling Pathway:we used the same way which as mentioned above to breed them, then killed the rats the day after two weeks. those model groups would be obtained two weeks later and observed brain organization Hippocampus japonicus CA1,CA3,DG and BLA section CREB. P-CREB. ERK1/2. P-ERK cell number and immune positive neuron.Results:1 Compared with the regular group, during making models, the rats are losing weights. There were big differences from week one to week four (P<0.001); blood serum decrease as well, and also the big differences between week two and week three (P<0.05) as well as week two to week four (P<0.05). Plasma ACTH and blood serum CORT increased between week two till week four. Blood serum and plasma increased at week three. Spleen and thymus gland decrease at week four (P<0.01,P<0.001) and immuno-cell factor IL-2 decrease at week four. 2 Compared with regular group, during making model, the model group increasing weight slowly and big differences (p<0.001); horizontal movement and vertical movement during of Open field test decrease (p<0.01), blood serum CORT and plasma ACTH increases (p＜0.001); plasma MTL and blood serum GAS decreases (P＜0.05); spleen weight and thymus gland weights decrease (p<0.01, p<0.001); blood serum IL-2 decrease a lot (p<0.01)Compared with model group, CHSGS group increases a lot weight (p<0.001), so does the SJZT group (p＜0.001); horizontal score and vertical score of CHSGS group increases and the stay time in the central form and rats'shit decreased. SJZT group encountered the similar situation but make no differences (p>0.05); the immobility time of forced swimming of CHSGS group shortened and The SJZT group didn't and made no obvious differences (p>0.05); the amount of blood serum CORT and plasm ACTH from CHSGS group decreased (p<0.01, p＜0.001), the amount of blood serum CORT and plasm ACTH from The SJZT decreased a bit and made no obviously differences (p>0.05); the amount of blood serum GAS and plasm MTL from CHSGS group increased (p>0.05), however, the amount of blood serum GAS and plasm MTL from The SJZT group tend to increase as well, and made no obviously differences (p>0.05); the weight of spleen and thymus from both group increased (p<0.05, p<0.01);and blood serum IL-2 from group increased as well (p<0.05).Compared with regular group, body weight from CHSGS group increased but with no differences (p>0.05), body weight from SJZT group increased slowly and with a lot different (p<0.001>); horizontal scores and vertical scores from both group increased, stay time in central form and shit amount decreased, but made no obviously differences (p>0.05); the immobility time of forced swimming from CHSGS group decreased obviously (p>0.05), made no obviously differences. The immobility time of forced swimming from The SJZT group shortened a lot and made great differences (p>0.001); the amount of blood serum CORT and plasma ACTH from both group decreased and made no obvious differences (p>0.05); the amount of blood serum GAS and plasma MTL from both group increased and made no obvious differences (p>0.05); the spleen weight and thymus gland weight from both group increased and made no obvious differences (p>0.05); the amount of blood serum IL-2 increased and made no obvious differences (P<0.05).3 Compared with regular group, CA3, DG section from Hippocampus japonicus and BLA ERK1 amount of positive cell, the positive area from CA3 section ERK1 of Hippocampus japonicus decreased a lot. CA3, DG section from Hippocampus japonicus and BLA ERK2 amount of positive cell, the positive area from CA3 section ERK2 of Hippocampus japonicus decreased a lot; CA3, DG section and BLA P-ERK positive cell amount from Hippocampus japonicus, DG section and BLA positive area decreased a lot.; each section of Hippocampus japonicus from model group and BLA CREB, P-CREB amount of positive cell, the positive area decreased a lot.Compared with model group, the Hippocampus japonicus CA3, DG section and BLA ERK1/2 amount of positive cell from CHSGS, and Hippocampus japonicus CA3 section ERK1/CA and BLA ERK2 increased obviously, and the same thing happen to the SJZT Group but made no obvious differences. Hippocampus japonicus CA3, DG section and amount number of BLAP-ERK positive cell, Hippocampus japonicus DG section and BLA positive area increased, those figures from The SJZT tend to increase as well but with no obvious differences;the amount of BLA CREB, P-CREB positive cell, positive area from each section of Hippocampus japonicus increased obviously, the same thing happened to The SJZT group but didn't make a lot differences.Compared with regular group, the above figures from CHSGS group and SJZT group has no differences.Conclusion:1 This experiments focus on internal secretion, immunization, stomach and Intestines to observe the way to produce the different circumstances of stagnation of liver-QI on different period, which indicates that it only takes two weeks that can produce stagnation of liver-QI rats'model.2 CHSGS can make scores by increasing horizontal movement and vertical movement of stagnation of liver-QI rats. It can improve the stagnation of liver-QI rats' behavior by extending stay in the central form, increasing the shit amount, shortening the forced swimming time.3 CHSGS has the power of anti stagnation of liver-QI; it is probably related to improve the rats'blood ACTH and CORT, which restrains HPA axle. CHSGS can also adjust stomach and intestines by improving plasm MTL and GAS. It mainly improves immunization system by increasing the amount of stagnation of liver-QI rats'blood serum IL-2, spleen weight and thymus gland weight, from which improved the power of anti stagnation of liver-QI.4 CHSGS has the power of anti stagnation of liver-QI; CHSGS can protect the damaged nerve cell; improve the brain functions, relieving stagnation of liver-QI rats' symptom by improving Hippocampus japonicus CA3, DG section and BLA ERK1/2, P-ERK signal pathway activities;it is probably related to the improvement each section of rats'Hippocampus japonicus and BLA section CREB, P-CREB. It boosts the recovery of those stressed damaged nerve cell, improves the stagnation of liver-QI rats'symptom, moreover, and improves the power of anti stagnation of liver-QI.5 The way of relieving depression of the liver-qi can relieve depression of the liver-qi by improving the blood ACTH and CORT, which restrains HPA axle,improve the plasm MTL and GAS, improving immunization system by increasing the amount of stagnation of blood serum IL-2, spleen weight and thymus gland weight, improving Hippocampus japonicus CA3, DG section and BLA ERK1/2, P-ERK signal pathway activities, improving each section of Hippocampus japonicus and BLA section CREB, P-CREB.