Study On Variety And Quality Evaluation And Population Ecology Of Acanthopanax Giraldii Harms

The stem bark of Acanthopanax giraldii Harms was a habitually used species in Sichuan Province and a special medicinal material of Qiang ethnic minority in upstream of min river. It was recorded in Standard Chinese Herbal Medicines in Sichuan Province of 1987 edition, with effectiveness of expelling wind-evil and removing dampness, easing tension of joints, strengthening muscle and bones, to cure the disease such as wind-cold-damp arthralgia, powerless of knee and so on. Modern research has shown its effectiveness of anti-tumor and enhance the immunization. Therefore it shows great value of development. Nowadays, there's still no systematical quality control standards for A. giraldii Harms. With sustained growth in market demand and disorder cutting, there was a downward trend in its production.The article systematically carried on studies as follows:Studied the species of A. giraldii Harms. The result has shown that A. giraldii var. hispidus belong to A. giraldii Harms. The deference between them was within specie level. So did the plant of "white cortex acanthopanacis".The article firstly established a systematical quality control standards for A. giraldii Harms. Distinguishing, inspecting, setting up determination methods and HPLC fingerprint for 56 batches of stem barks. Found out the microscopic characteristics. Determined the limit standards for moisture, total ash and acid-insoluble ash Suggested the content of water soluble extraction (hot-dip method) should not be less than 12.9 percent, content of volatile oil should not be less than 0.30%, content of polysaccharides should not be less than 10.9%, content of eleutheroside E should not be less than 0.05%, content of chlorogenic acid should not be less than 0.30%. Analyzed HPLC fingerprint of the stem bark and identified twenty chromatographic peaks as common peaks, the No.10, No.11 and No.16 peaks was chlorogenic acid, eleutheroside B and eleutheroside E respectively. And the greatest peak was the eleutheroside E.The article firstly systematically compared the active components between the xylem and bark of stem. Determined and compared 10 batches of xylem and it's bark respectively. The content of eleutheroside E, chlorogenic acid and polysaccharides in xylem was 1/4 or nearly equal to that of the bark. The content of eleutheroside E in old xylem was even much higher than the it's bark. There was high similarity between their HPLC fingerprint. Suggested to reserve the xylem for medicinal use so that enhancing the utilization rate of A. giraldii Harms. The article firstly carried out systematical pharmacognostical study for leaves of A. giraldii Harms. Identified, pretested the chemical composition and determined the active components of 18 batches of leaves samples. Meanwhile, established its HPLC fingerprint. Suggested the content of hederagenin should not be less than 0.60%, the chlorogenic acid should not be less than 0.50%。Analyzed its HPLC fingerprint and identified 17 common chromatographic peaks, identified the No.8 peak was chlorogenic acid. Compared the fingerprint of leaf and bark from the same source, there was big difference between them, so the leaves of A. giraldii Harms has potency to be developed into a new medicinal part.The article firstly investigated the population ecology of A. giraldii Harms from four aspects:community, population, breeding and active components. Managed to explain the ecological amplitude, ecological succession process and interdependence between populations. Systematically studied the correlation within population characteristics, yield index and environmental factors. And analyzed the correlation between environment factors and accumulation of medicinal effective ingredients in bark and leaf of A. giraldii Harms.The growth and yield index were significantly correlated with several ecology factors such as forest canopy density, available potassium and aspect. The main reproductive strategy was clone breeding. The tender seedlings was adapt to shading environment and independent of soil fertility in some degree. The study above could provide first-hand information for the introduction and domestication of A. giraldii Harms. And could guide its introducing region choosing, seedling breeding and management.To sum up, the paper established quality control system of A. giraldii Harms and studied its population ecology. The research has far-reaching significance for establishing quality control standards, comprehensive assessment of cortex acanthopanacis, impelling deep development of the A. giraldii Harms and its sustainable utilization. The study could also provide reference for introduction and domestication of other medicinal plants in plateau.