Effect Of Activating Blood Circulation,Resolving Damp And Tonifying Liver And Kidney On Hepatic Cirrhosis

Purpose: From the theory of traditional Chinese medicine, we know that the postion of hepatical cirrhosis lies in the liver, spleen and kidney. Former doctors raise the gist of "it's easy to cure the yang-deficiency, while hard to yin-deficiency", it is reported that the syndrome of hepaticrenal yin-deficiency is an important pathogenesis, and also is a regular and stubborn syndrome, and this syndrome is easy to become hepaticalencephalopathy and bleeding than others. We are convinced of the pathogenesis of the hepatic cirrhosis is heat interwinding damp, and deficiency of liver and kidney. So activating the blood circulation and supplementing the liver and kidney is established. The compound drugs landou hugan granules is from the professor zouliangcai. After the clinical application, the effect is exact. The clinical part of this project is to declare the way of this compound to cure the hepatical cirrhosis, experimental part discuss the compound's effect on the hepatic fibrosis and morphologic pathology. And we can make the basis of treating hepatic cirrhosis.Methods: Randomized controlled trial in accordance with the principles of clinical research of the 180 cases of hepatitis observed in patients with liver cirrhosis, the control group given conventional treatment of western medicine, including lowering the liver enzymes, diuretic, dispelling jaundice, supplementary measures such as albumin; treatment group in the general treatment of the basis of Western medicine , the use of Landou Hugan granules, a major component drugs Herba Lycopi , black bean, fructus broussonetiae, fructus liquidambaris, Cyathnla capitata Moq, herba artemisiae, Lagenaria vulgaris Ser, thick fried 100ml, morning and evening service hours, treated for 6 months. Observed clinical signs and symptoms, liver function, liver defibrase indicators serological examination and morphological changes of the image.Experimental study on the use of modern experimental techniques, the use of pure Chinese medicine granules Landou Hugans on experimental hepatic fibrosis in 140 SD rats, prescription from Herba Lycopi , black bean, fructus broussonetiae, fructus liquidambaris, Cyathnla capitata Moq, herba artemisiae, Lagenaria vulgaris Ser, concentration of agents 2.64g/ml. Experiment is divided into two parts, namely, Landou hugan granules prevention and treatment of drug use, selection of colchicine as a control, application inspection serum biochemistry, pathology and immunopathology of conventional detection techniques, observation of the Landou Hugan granules prevention, treatment efficacy of hepatic fibrosis and to explore its mechanism.Result: Clinical studies have shown that Landou Hugan granules can significantly reduced serum transaminase activity, a significant reduction inγ-globulin to improve the A/G ratio, the two groups before and after treatment, the clinical symptoms and signs, liver function, serum indicators of liver defibrase and morphological examination improvement in varying degrees, the two groups after treatment, the difference was statistically significant. Treatment group 24 cases of cure (27%), improvement in 49 cases (54%), ineffective in 17 patients (19%), total effective rate of 81.1%. The control group cured 19 patients (21%), improvement in 34 cases (38%), 37 cases of invalid (41%), the total effective rate 58.9%. Efficacy of the treatment group than the control group, two groups by chi-square test, the difference was statistically significant (P <0.05). Landou Hugan granules at the same time can reduce the increase in patients with HA, LN, IV collagen, and difference between the time of before and after treatment were statistically significant (P <0.05).Experimental results show that: the prevention of high-dose group, middle dose group prevention, prevention of low-dose group, the prevention of colchicine group and model group I, ALT, AST were statistically significant differences (P <0.05), more than indicators are reduced to varying degrees, but the difference was not statistically significant, Landou Hugan granules in rats acute liver injury have some effect in prevention and there is a certain degree of dose-effect relationship. Indicators of the impact of liver fibrosis, prevention of high-dose group, middle dose group prevention, prevention colchicine group compared with the normal group, with the exception of LN, the more was no significant difference in the prevention of middle dose group, low-dose group to prevent fibrosis indicators increased in varying degrees; Compared with model group, the prevention of high-dose group, middle dose group prevention, prevention colchicine group were reduced to varying degrees, HA (P <0.01), PIIINP (P <0.05), There were significant differences, LN was no significant difference; the prevention of low-dose group of HA (P <0.01), the difference was statistically significant, the prevention of low-dose group LN, PIIINP lower, but the difference was not statistically significant.Experimental drugs in the treatment of high-dose treatment groups, treatment dose, treatment of low-dose group, treatment colchicine group had restoration of liver function group, compared with model group II was no significant difference, CCl₄-induced experimental rat liver injury model, after the cessation of CCl₄-induced, self-restoration of liver function, ALT, AST to resume quickly, ALB, TP restoration of slow repair in rat liver was comparatively strong, so the treatment group and model group and the difference is not statistically significant. When compared to hepatic fibrosis, HA compared with the normal group had no significant differences in comparison with the model group I significantly different (P <0.01), LN, P III NP than the normal group was significantly higher (P <0.05), with the model group I no significant difference, suggesting that CCl₄-induced rat liver fibrosis model drug after cessation of self-recovery experiment in the model group than in model I II group, despite the improvement in fibrosis index, the overall difference with normal group statistics significance; the treatment of fibrotic markers in each group has a different degree of reduction compared with the model group II, the treatment of high-dose group, the treatment dose, treatment of low-dose group, the treatment of fibrosis colchicine group of indicators in each group were improvement, HA (P <0.01), treatment of high-dose group, the treatment dose group, treatment group colchicine group LN (P <0.05), treatment of high-dose group, treatment colchicine group P III NP (P <0.05), There were significant differences, Portland Hugan bean granules of rat liver fibrosis have a better therapeutic effect and dose-effect relationship exists. Observed that the pathomorphology of Landou Hugan granules on carbon tetrachloride-induced rat liver fibrosis model was better prevention, treatment effect, can inhibit the expression of Smad4 and reduce the deposition of ECM.Conclusion: Landou Hugan granules can improve hepatitis symptoms in patients with liver cirrhosis, catabatic liver function and improve the liver fibrosis, experimental study of Landou Hugans can significantly improve liver function of the model CCM rat model of liver fibrosis, liver fibrosis indicators may be related to Landou Hugan granules inhibiting the expression of Smad4 and reduce the deposition of the ECM.