Study On Effective Materials Of Fritillaria Monantha Migo And Preparation Of Beixinggan Double-deck Buccal Tablet

Objective: To study some key factors on developing Fritillaria monantha Migo ,such as effective components,collecting time& dry process of crude bulbs, quality control method and pharmaceutiacl way of covering bitter taste.Methods: (1)The chemical constituents of Fritillaria monatha Migo have been studied systematically by chromatography method and spectroscopy analysis;(2) In vitro trachea strips model has been established and applied to study the mechanism of some active compounds of Fritillaria monatha Migo; (3) The method of HPLC-ELSD has been developed to determinate peimisine and peminine simultaneously; Initial studies have been conducted on collecting time of Fritillaria monantha Migo bulb by HPLC-ELSD fingerprint technique. (4)Different sweeters and preparetion process has been applied to make buccal tablet.Results:(1) twenty-six compounds are seperated from Fritillaria monantha Migo bulbs, among them twenty compounds having been dentified. They are determined as peimisine(FA₁),peminine(FA₂),peimine(FA₃),pengbeisineA(FA₄),pengbeisineB(FA₅),pengbei mineA(FA₆),pengbeimineB(FA₇),pengbeimineC(FA₈),ent-kauran-15-en-17-ol(FD₁),campesterol(FD₂),stearicacid(FD₃),ent-kauran-16β, 17-diol(FD₄),ent-16,17-epoxy-kauran-3β-ol(FD₅),daucosterol(FD₆),pengbeimine(FD₇),isoverticine(FD₁₀),fritillaziebinol(FD₁₂),ent-kaurane-3β, 16β, 17-triol·H₂O (FD₁₃),ent-16α-methoxy-kauran-17-ol (FD₁₄),ent-kauran-15-en-6α,17-dio (FD₇).The compounds pengbeisine A, pengbeisine B, pengbeimine A, pengbeimine B, pengbeimine C, pengbeimine D, and ent-16,17-epoxy-kauran-3β-ol are seven new compounds. (2)The results of pharmacological experiments show that the EtOH extracts can control the rat's cough from ammonia, and can increase the quantity of FenHong in the rat's trachea. In the mean while, the CHC1₃â…¡extracts can control the rat's cough from ammonia, and the BuOH extracts can increase the quantity of FenHong in the rat's trachea. Pharmacological studies further reveal that peimisine and peminine can release the tracheal smooth muscle contraction caused by Ach through reaction on M receptor. This result demonstrates that peminine can be selected as index to control the quality of crude Fritillaria monantha Migo bulb and Chinese patent medicine which contain Fritillaria monantha Migo bulb.(3) The best collecing time of Fritillaria monantha Migo bulb is of 4. Dry process of Fritillaria monantha Migo bulb also been researched ,reletively better dry conditions are established.(4)Beixinggan double-deck buccal tablet has been prepared.Conclusions: Fritillaria monatha Migo bulbs contain chemical components which can relieve cough and antiasthma, Peimisine and Peimine are two main active ingredients. Peimine can be choosed as quality control ingredient and can be assaied by HPLC-ELSD. It is demenstrated that taking double-deck buccal tablet process can effectively cover bitter taste of Chinese patent medicine.The thesis get the financial supports from the National Natural Science Foundation of China(30160097).