| Objective To study the effect of xiefu zhuyu decoction against myocardial ischemia injury and the possible functional pathways. Methods Rats were divided into four groups:sham operating group, model group,xiefu zhuyu decoction group,diao xinxie kang goup.The model of myocardial ischemia injury was produced by ligaturing anterior descending branch of left coronary artery for 60 minutes in rats.The contents of SOD,MDA,LDH,CK,apoptosis,expression of Bax,Bcl-2,ICAM-1 and Caspase-3 after ischemia and the effects on above items of xiefu zhuyu decoction were observed.Results(1 ) Xiefu zhuyu decoction could significantly reduce the content of MDA in ischemia injury myocardium and blood of rats(P＜0.05 ),while obviously increasing the activity of SOD in ischemia injury myocardium and blood of rats(P＜0.05 ). (2 ) Xiefu zhuyu decoction could significantly decrease the levels of CK and LDH in the rats blood of myocardial ischemia injury(P＜0.01,P＜0.05 ). (3) Xiefu zhuyu decoction could significantly reduce the number of myocardial cell apoptosis(P＜0.01)(4) Xiefu zhuyu decoction could obviously reduce the expression of Bax(P＜0.01),while significantly promoting the expression of Bcl-2 in ischemia myocardium(P＜0.05 ).(5 ) Xiefu zhuyu decoction could significantly reduce the expression of ICAM- 1 and Caspase-3 in ischemia myocardium(P＜0.05,P＜0.01).Conclusion Oxygen free radical,enzyme,apoptosis,cell adhesion molecule,cysteinyl aspartate specific proteinase participate myocardial ischemia injury.Xiefu zhuyu decoction has the effect against myocardial ischemia injury.This effect of Xiefu zhuyu decoction could be carried out by the following pathways:reduce the injury by oxyen free radial,decrease the out-leaking of enzyma,decrease the number of myocardial cell apoptosis,reduce the expression of Bax,,ICAM-1 and Caspase-3,improve the expression of Bcl-2. By the above ways,xiefu zhuyu decoction could effectively prevent the myocardial ischemia injury. |
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