Clinical Observation On Treatment Of Benign Prostatic Hyperplasia With Chinese Traditional Medicine Qian Long Tong And Probe Into The Therapeutic Mechanisms

Objective:The study was from the clinical cure obervation and the animal experiment to probe into the curing and therapeutic mechanisms of Qian Long Tong Capsule,in order to support the prescription with scientific content and make it come into effect as soon.Clinical investigationMethods:Sixty patients of Benign prostatic hyperplasia were randomly divided into 2 groups,the treatment group(30 patients) and the control group(30 patients),the former treated by Qian Long Tong Capsule,the latter treated by long Bi Shu Capsule,we observed the patient urination's symptoms,living quality and so on,we tested the patient's prostatic volume of Benign prostatic hyperplasia using Brightness mode ultrasonic examination and Qmax uroflowmetry,Average uroflowmetry using uroflowmeter.Results:Qian Long Tong capsule,could improve Qmax uroflowmetry, reduce the excel urine and lessen the volume of the prostate comparing with the control group there were significant difference between two groups(P＜0.01). Conclusion:The clinical cure observation show Qian Long Tong Capsule could improve Qmax uroflowmetry,reduce the excel urine and lessen the volume of the prostate.Experimental study:Methods:To study the histologic changes of Benign prostatic hyperplasia, we used lightmicroscope and electronmicroscope,the influences of positive expression for FGF₇,TGFβ₁,VEGF,α-SMA,FGF₇mRNA and TGFβ₁ mRNA were evaluated by the immunohistochemistry and in situ hybridization.All the pictures were done with the MIAS images analysis system.Results:1.Qian Long Tong capsule,varied dose group,Long Bi Shu group rats prostate waterish-weight,volum,prostatic index,gland,glandular epithelium and interstitium area were all less than model group(p＜0.01). The high dose group's were less than Long Bi Shu(p＜0.05).2.Qian Long Tong Capsule varied dose group,Long Bi Shu capsule group rats prostate FGF₇gray-tolerance-value,positive cell areas were significantly less than model group(p＜0.01),the high,middle dose group, Long Bi Shu group integral-spectral-density significantly less than model group(p＜0.01).The high dose group's FGF₇gray-tolerance-value, integral-spectral-density were significantly less than Long Bi Shu group (p＜0.05),and the positive cell areas were significantly less than Long Bi Shu group(p＜0.01).3.Qian Long Tong Capsule low dose group rats prostate FGF₇mRNA gray-tolerance-value were significantly less than model group(p＜0.05), high,middle dose group were significantly less than model group(p＜0.01). Qian Long Tong Capsule varied dose group,Long Bi Shu group rats prostate FGF₇mRNA integral-spectral-density significantly less than model group(p＜0.01).The middle dose group,Long Bi Shu group rats prostate FGF₇mRNA positive cell areas were significantly less than model group(p＜0.05).The high dose group positive cell areas were significantly less than model group(p＜0.01),and FGF₇mRNA gray-tolerance-value, positive cell areas were significantly less than Long Bi Shu group (p＜0.05).The high,middle dose group FGF₇mRNA integral-spectral -density significantly less than Long Bi Shu group(p＜0.01).4.Qian Long Tong Capsule high,middle dose group,Long Bi Shu group rats prostate TGFβ₁ gray-tolerance-value,positive cell areas integral-spectral-density were significantly less than model group(p＜0.01), The low dose group TGFβ₁,integral-spectral-density,positive cell areas were significantly less than model group(p＜0.05).The high dose group TGFβ)₁ gray-tolerance-value,integral-spectral-density,positive cell areas were significantly less than Long Bi Shu group(p＜0.05).5.Qian Long Tong Capsule low dose group gray-tolerance-value were less than model group(p＜0.05),high,middle dose group average gray-tolerance-value,integral-spectral-density were significantly less than model group(p＜0.01).Qian Long Tong Capsule varied dose group,Long Bi Shu group positive cell areas were significantly less than model group(p＜0.01),High dose group,gray- tolerance -value,positive cell areas were significantly less than Long Bi Shu group(p＜0.05).6.Qian Long Tong Capsule high,middle dose group,Long Bi Shu group gray-tolerance-value were significantly less than model group(p＜0.01).Qian Long Tong Capsule varied dose group,Long Bi Shu group integral-spectral-density, positive cell areas were significantly less than model group(p＜0.01).The high dose group gray-tolerance-value,integral-spectral-density,positive cell areas were significantly less than Long Bi Shu group(p＜0.05).7.Qian Long Tong Capsule high,middle dose group,Long Bi Shu group gray-tolerance-value,integral-spectral-density were significantly less than model group(p＜0.01).Qian Long Tong Capsule varied dose group,Long Bi Shu group positive cell areas were significantly less than model group(p＜0.01).The high dose group gray-tolerance-value were significantly less than Long Bi Shu group(p＜0.05),integral-spectral -density were significantly less than Long Bi Shu group(p＜0.01),positive cell areas were close to the Long Bi Shu.Conclusions:The study concluded the curing and operating mechanisms of Qian Long Tong Capsule from the clinical cure obervation and experimental study.Clinical investigation show,Qian Long Tong Capsule could improve Qmax uroflowmetry,reduce the excel urine and lessen the volume of the prostate,the experimental study found out it could reduce rats prostate waterish-weight,lessen volum,depress prostatic index lessen glandular epithelium and interstitium area,depress the expression of FGF₇, TGFβ₁,VEGF,α-SMA,FGF₇mRNA and TGFβ₁ mRNA.Meanwhile it could improve epithelial cell vacuolation of mitochondrion,glandular interstitium neutrophil infliltration,endoplasmic reticulum hydrops and dilatation,intracavitray secretion adding and many pathologic changes.