Induction Of Innate And Adaptive Immune Responses To Hepatitis B Virus

Objective: To detect the immune reaction of HBV PreS1 peptides with different genotype and evaluate the potential influence for host immunity of the peptides as a new entry inhibitors for the treatment of acute and chronic hepatitis B virus infection.Methods: 1. Immunization of Balb/C mice with different methods. 2. Measurement of the HBV PreS1 peptides induced specific antibody response and the crossing reaction with ELISA. 3. Detection of the Anti-PreS1 antibody binding with the purified hepatitis B virus particles with ELISA. Detection of the antibody recognizing purified virus particles and its role in virus clearance with immunoprecipitation, Real Time PCR and so on. 4. Detection of specific Anti-preS1 peptides antibody in the serum of chronic HBV infectious patients in different immunization status with ELISA. 5. Analysis of virus genotype of different patient background with PCR.Results: 1. HBV preS1 peptides could induce specific antibody in Balb/C mice. 2. The Anti-preS1 for different genotype could have crossing reaction. 3. The Anti-PreS1 could recognize purified hepatitis B virus particles and bind with them.Conclusion: HBV PreS1 peptides could induce the specific Anti-PreS1 that could recognize hepatitis B virus particles and bind with them, which indicated that the specific antibody have ability of clearing virus to a certain extent. As the peptides could induce production of specific antibody, it maybe not fit for clinic long-term treatment.