| Partâ… Literature Review and StudyWe studied the knowledge of Modern Medicine and Traditional Chinese Medicine(TCM) to radiodermatitis.Then We discussed the basic and significance of the research.Partâ…¡Clinical StudyObjective:To obsever the therapeutic efficacy of Jinhu Cream in preventing and treating acute radiodermatitisMethods:60 patients with Head and Neck Cancers were divided randomly into two groups.In Group A(as a treatment group),30 patients were treated with Jinhu Cream since the first radiotherapy session.In Group B(as a control group),30 patients were given general and health education since the first radiotherapy session.The therapeutic efficancy of the two groups were evaluated after two course of treatment.Results:The rate of the skin-reaction was 100%in the patients of both groups. A mild radiation reaction(gradeâ… andâ…¡) occurred as follows: 70.0%(21/30cases) in Group A and 40.0%(12/30 cases) in Group B.A severe reaction(grade-â…¢) radiation reaction developed in 30.0%in Group A,and 60.0%in GroupB.There was a significant difference(P<0.05) between the two groups.Concerning the degree of the skin response before the patients received a dose of 40 Gy,the radiation reaction emerged in 26.7%of the cases in Group A,and in 60%of the cases of Group B.The mean time for curing radioactive dermatitis,Group A and B was 7.31 and 9.68 days.There was a significant difference(P<0.05) between the two groups.Group A had the same quality of life with Group B.Jinhu Cream had no severe adverse reaction.Conclusion:Jinhu Cream can effectively prevent acute irradiator induced dermal injury.It can alleviate the patients' suffering and improve the quality of life,so as to ensure uneventful radiotherapy.Partâ…¢Animal ExperimentObjective:By the animal experiment,to observe the curative effect of Jinhu Cream on on skin ulcer induced by radiation in rats and the expressions of Ku70/Ku80 mRNA in acute irradiated skin injury in rats,the result of which would be based for further clinical research.Methods:In this model the radiation injury on hip skin of female SD rats was induced by 6MeV electrons from an electron linear accelerator.Experimental animals were divided into 4 groups,Group A(the model group treated with Sodium Chloride),Group B(the control group treated with Biafine cream) and Group C(treated with Jinhu cream) and Group D(the blank group).After treated with 14 and 30 days,the pathological changes was observed macroscopically and by light and electron microscopy.The irradiated skins of the rats were examined by real-time quantitative PCR to detect the expressions of Ku70/Ku80 mRNA.Results:The rate of the skin-reaction was 100%in rats of both groups.There was no significant difference(P>0.05) between three groups.The morphologic changes of Group A,B,C wasn' t obviously under light microscope after 14 days. There was significant difference in the pathological changes after 30 days. It could observe part of wound repair,the proliferation of fibroblasts and collagen fiber in Group B and C whlie obviously wound lesions,epidermis and dermis tissue necrosis,a large number of inflammatory cell infiltration in Group A.Under electron microscope,the swelling of mitochondria and endoplasmic reticulum,apoptosis in basal cells,swelling,pyknosis,cavity narrowing and thrombosis in microvessels,disruption of collagenous fibrils and apotosis in fibroblasts can be observed in Group A,B,and C after 30 days. The contents of Ku70 mRNA in the skin of Group C were significantly decreased than that in Group A(P<0.05) after 30 days.The contents of Ku80 mRNA in the skin of Group C were significantly increased than that in Group A(P< 0.05) after 30 days.Conclusion:Jinhu Cream can effectively cure radiation skin ulcer,and the effect is especially significant on the ulcer induced.It can promote the composition of collagen and increase the expressions of Ku70/Ku80 mRNA,then improve wound healing.And integrity of the skin and damaged skin of the rats did not have acute toxicity.
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