| At present researchers extract sixty various chemical constituents from scutellaria,and the main are flavonoids,such as baicalin,baicalein,wogonoside,wogonin,neobaicalein.Especially baicalin is high content and is a quality standard of testing scutellaria in pharmacopoeia.But there are difference content of total flavones and main active component in different sources scutellaria. Various substance basis is a possible reason of scutellaria having different pharmacological action.Scutellaria have wide anti-virus effect.It's pharmacodynamic study achieve a level of cellular and molecular,and research objects include water decoction,total extraction,single-component,contrast of several ingredients pharmacodynamic study.But there is a blank about integration effect of effective components,coexistence and reasonable ratio of effective components may be substance basis of pharmacodynamic actions.Therefore studying scutellaria reasonable ratio embodying effect quality can provide a reference to other chinese medicinal materials study.Almost every year there is influenza epidemic outbreak of local area,and influenza incidence is the highest in China..Influenza vitus is easy to be a variation,which brings great difficulty for vaccine preventing.So there isn't specific remedy now.Traditional chinese medicine(TCM) accumulated abundant theories and practical experience.Further study on TCM anti-influenza virus could promote human health.This research topic compared pharmacodynamics difference of different sources scutellaria on anti-influenza virus by using TCM theory and chinese drug pharmacology. Meanwhile on the basis of extracting main active component and analyzing spectrum-effect relationship,making experiments of active component ratio,which found out the best substance basis on anti-influenza virus.Besides we had study on mechanism and expected to provide experimental basis for scutellaria quality detection and innovative drugs study.Method:1 ICR mice had been divided into blank,model,ribavirin,He Bei ethanol extract,He Bei aqueous extract,Shah Xi ethanol extract,Shan Xi aqueous extract,Da Lian ethanol extract and Da Lian aqueous extract at random.After being anesthetized by ether,blank was dropped saline in nose and other group was dropped A/FM/1/34(FM1) in nose.After infection an hour the mice were treated,administration would last six days.Observed the effect of different sources extracted scutellaria on the death protection,lung index and hemagglutination titer of FM1 infected mice.2 Analyzed spectrum-effect relationship through grey correlation analysis method and high performance liquid chromatography,then we determined the best component of ratio and verified it was right or wrong.ICR mice had been divided into blank,model,ribavirin,He Bei aqueous extract and scutellaria element ratio at random.After being anesthetized by ether,blank was dropped saline in nose and other group was dropped FM1 in nose.After infection an hour the mice were treated,administration would last six days.Observed the effect of different sources extracted scutellaria on the death protection,lung index and hemagglutination titer of FM1 infected mice.3 ICR mice had been divided into blank,model and scutellaria element ratio at random.After being anesthetized by ether,blank was dropped saline in nose and other group was dropped FM1 in nose.After infection an hour the mice were treated,administration would last three days. Observed tissue morphology structure change of mice lung by light and electron microscopic,lung tissue apoptosis by TUNEL,expression content of Bcl-2,Bax,Fas,FasL by immunohistochemistry,serum expression change TNF-αby ELISA and Caspase-3,NF-κB p65 by Western Blotting.Result:1 There were very significant difference from ribavirin,He Bei aqueous extract to model on mortality.Significant difference existsed in He Bei ethanol extract,Shan Xi aqueous extract,Da Lian ethanol extract and model on mortality.There were difference from Shan Xi ethanol extract,Da Lian aqueous extract to model on mortality.There were no difference from ribavirin,He Bei aqueous extract to blank on average survival days,however difference existsed in other extracted scuteltaria and blank.There were very significant difference from ribavirin,He Bei aqueous extract,Shan Xi ethanol extract,Da Lian ethanol extract and Da Lian aqueous extract to model on lung index,significant difference from He Bei ethanol extract,Shan Xi aqueous extract to model on lung index.There were no difference from He Bei aqueous extract,Shan Xi ethanol extract and Da Lian aqueous extract to ribavirin on lung index and difference existsed in other extracted scutellaria.There were very significant difference from ribavirin and each extracted scutellaria to model on hemagglutination titer,without difference from He Bei aqueous extract and Da Lian aqueouS extract to ribavirin on hemagglutination titer,but difference existsed in other extracted scutellaria and ribavirin.2 Scutellaria element ratio i ncluded Baicalin,wogonin and baicalein through grey correlation analysis method.There were significant difference between scutellaria element ratio and model on mortality,average survival days,lung index and hemagglutination titer.There were no difference between He Bei aqueous extract and scutellaria elemen ratio.3 The lung of blank mice were pink,containing air and without real variable area by general observation.The lung of model infected mice were dark brown and with two lobar real variable areas.Model infected mice had severe interstitial pneumonia lesions including lung epithelial cells degeneration,apoptosis,infiltration of inflammatory cells and so on through light microscope and electron microscope.Lesion degree of scutellaria element ratio were better than model and scutellaria element ratio lung tissue closed to normal.There were significant difference between scutellaria element ratio and model on apoptosis index,Bcl-2,Bax,Fas,FasL,Caspase-3,NF-κ3 p65 by TUNEL,ICH,ELISA and Western Blotting.Conclusion:1 The system on material basis of chinese medicine effect has complexity character including complexity of chemical constituents,interaction and human,We should study it by complex science theory,method and technology,pharmacology,drug chemistry,mathematics computer and so on.2 Different sources extracted scutellaria both had good effect on the death protection,lung index and hemagglutination titer of FM1 infected mice.3 Scutellaria element ratio had good effect on the death protection,lung index and hemagglutination titer of FM1 infected mice.4 Not only Baicalin but also wogonin,baicalein were important sustance basis anti-influenza virus in scutellaria.5 Scutellaria element ratio could significantly improve pathological change of FM1 infected mice lung,decrease apoptosis index,up-regulate Bcl-2 expression content and decrease Bax,Fas,FasL,TNF-α,Caspase-3,NF-κB p65 expression content of FM1 infected mice.It would antagonize FM1 by inhibiting apoptosis and preventing virus proliferation. |
