Experimental Study Of The Physiological Mechanisms Of Lung Autumn

Theory of "Yinyang of five Zang-organs and four seasons" express that man is an integrated whole,and man and nature is an integrated whole.Theory of "Lung correspondence to autumn "is the important content of theory of "Yinyang of five Zang-organs and four seasons".The researches on theory of "Lung correspondence to autumn " mainly focus on how lung adapt the changes of nature to maintain the balance of physiological function.Recently,seasonal rhythm influencing on man's physiology and pathology becomes an interesting direction for many researchers. Studying on the seasonal climate influencing on physiological function is of great importance to clinical diagnosis,theory and preventing diseases.1ObjectiveTaking the idea of"lung correspondence to autumn" in TCM as a breaking point, we research on the seasonal changes of lung cell signal transduction to discuss the truth of theory of "Yinyang of five Zang-organs and four seasons",which can enrich the scientific methods of theory of "Yinyang of five Zang-organs and four seasons". Through this research,we can give some theoretic and experimental proofs on clinical diagnosis,theory and preventing diseases.2Methods2.1Theoretical methodsThe methods of the documentary materials and logical analysis were used to explain and analyze the physiological mechanism of "lung correspondence to autumn" and the relationship of "lung correspondence to autumn" and cell signal transduction.It showed that "Zang Fu proper to nature" had theoretical basis,and enriched the theoretical connotation of "Yinyang of five Zang-organs and four seasons".2.2Experimental methods2.2.1 Experimental animalsTwenty-four Wistar male rats,weighted 150-170g,brought from Beijing Weitong Lihua animal study center forty days before vernal equinox,summer solstice,autumnal equinox and winter solstice,were randomly divided into three groups:normal group,fake group and operation group(pinealectomy group).There were eight rats in every group.All rats received natural light,ate and drank freely,and were controlled under certain room temperature was 17℃±2℃in winter and spring,and it was 25℃±2℃in summer and autumn.Fake group and operation group are operated a month before vernal equinox,summer solstice,autumnal equinox and winter solstice.All the rats would be raised till vernal equinox,summer solstice,autumnal equinox and winter solstice,on these days at 8 o'clock at night,they would be beheaded.2.2.2 Test methodsTo assay the contents of G protein in lung,westen-blot was engaged.To assay the contents of cAMP cGMP in lung,ELISA was engaged.To assay the contents of IP₃ in lung,Radio-immunity was engaged.To assay the express of c -fos mRNA and c-jun mRNA in lung,Tissue in situ hybridization(TISH) was engaged.2.2.3 Analysis method:All the data were showed by mean±standard error.The differences of groups were tested by SPSS 11.0.The values were calculated with Duncan's multiple range test or Student's test.P＜0.05 meant obvious difference.3Results3.1 Results of theoretical study3.1.1 In autumn,lung can adjust itself to correspond autumn,it also can influence the other four zang-organs to adapt to the changes of the nature,and this is the physiology of theory of "Lung correspondence to autumn "3.1.2 After analysis of the pertinence between the modulating mechanism of 'Lung correspondence to autumn' and cell signal transduction theoretically in this study,we think they all emphasize on nature's influence on human being,and there are whole control mechanism which aimed at modulating.So we can from the point view of cell signal transduction accords with the essence of'Lung correspondence to autumn' in TCM.3.2 Results of experiments3.2.1 Seasonal change can influence cell signal transduction of lungIn normal stasis,the cell signal transduction have the character of seasonal changes.(1) Gq in lung of spring was lower than it of summer and autumn (P＜0.05),significantly lower than it of winter(P＜0.01);Gs in lung of spring was significantly lower than it of summer and winter(P＜0.01),Gs in lung of summer was significantly higher than it of spring and autumn,Gs in lung of autumn was significantly lower than it of summer(P＜0.01) and lower than it of winter(P＜0.05).(2)cAMP in lung of summer was higher than it of autumn(P＜0.05);cGMP in lung of spring was significantly lower than it of autumn(P＜0.01 ) and lower than it in winter(P＜0.05)(3)Seasonal change can not influence on Density mean of c-fos mRNA and c-jun mRNA in lung.But Per Area of c-fos mRNA in lung of spring was significantly lower than it of summer,autumn and winter(P＜0.01).Per Area of c-jun mRNA in lung of spring was significantly lower than it of summer,autumn and winter(P＜0.01 ), and if of summer was higher than it of winter(P＜0.05). 3.2.2Pineal gland can influence on the substances of cell signal transduction in lungIf we remove the Pineal gland,the substances of cell signal transduction in lung can changes:(1)Normal group of Gq in lung of spring was lower than operation group of it (P＜0.05).Fake group of Gq in lung of winter was significantly lower than it of spring and autumn(P＜0.01 ).Normal group of Gs in lung of summer was higher than operation group of it(P＜0.05).Fake group of Gs in lung of winter was significantly higher than it of spring,summer and autumn(P＜0.01).(2)Operation group of IP₃ in lung of autumn was higher than normal group and fake group of it(P＜0.05).(3)Normal group of Density mean of c-fos mRNA in lung of winter was lower than operation group of it(P＜0.05).Normal group of Per Area of c-fos mRNA in lung of summer and autumn was lower than operation group of it(P＜0.05).Normal group of Density mean of c-jun mRNA in lung of winter was lower than operation group of it(P＜0.05).Normal group of Per Area of c-jun mRNA in lung of autumn was higher than operation group and fake group of it(P＜0.01).Above all,Pineal gland can effect on the substances of cell signal transduction in lung.4 Conclusions4.1 Cell signal transduction in lung have seasonal changes..4.2 Pineal gland can effect on the substances of cell signal transduction in lung.4.3 The modulating mechanism of "Lung correspondence to autumn" maybe through the routine of Gs- cAMP-c-fos.cell in lung effect on the immune system.