Livers And Hearts Ning "effects And Mechanisms Of Prevention And Treatment Of Liver Fibrosis

Objective:To observe the preventive and therapeutic effect of "Ganxinning" capsule on experimental HF rats and the possible therapeutic mechanism,to provide the theoretic evidence for the clinic apply of"Ganxinning".Methods:57 SD rats were divided into 5 groups randomly,the normal group,the model group,the positive control group,the high dose group,and the low dose group.HF rat model was duplicated with Improved Composite Factor Method.Rats of the normal group and the model group were administered normal sodium with dose 10ml/kg,positive control group were administered Colchicine with the dose 0.125mg/kg.The high dose group and the low dose group were administered "Ganxinning" with the dose 26g/kg,6.5g/kg respectively.The liver pathology change of each group by HE coloration were compared and determination of liver tissue PDGF-BB expression 6 weeks later.The ALT,AST,and TBA in blood serum of experimental rats were detected by automatic biochemical analyzer.The HA,PⅢNP and LN were detdected by radio-immunoassay.The expression of caspase-3,bcl-2,PDGF-BB and bFGF were detected by immunohistochemical analyzer,Results:Compared with the model group,each treatment group has significant difference in the liver pathology integral(P＜0.05 or P＜0.01),and the low dose group is the most significant difference in the inflammation integral.Compared with the Colchicin group,each treatment group has significant difference too(P＜0.05).Compared with the control group,the ALT,AST and TBA of each group increased significantly(P＜0.05 or P＜0.01).And compared with the model group,these indexes of each treatment group decreased significantly(P＜0.01 or P＜0.05).these indexes between each treatment group showed no significant difference(P＞0.05).Compared with the control group,the LN,HA and PⅢNP of each group increased significantly(P＜0.05 or P＜0.01).And compared with the model group, these indexes of each treatment group decreased significantly(P＜0.01 or P＜0.05).The LN of lower dose TCM group was lower significantly than the Clochicin group(P＜0.05).these indexes between the other treatment groups showed no significant difference.Compared with the model group,the expression of bFGF of each treatment group decreased significant(P＜0.01).the expression of bFGF between each treatment group showed no significant difference(P＞0.05).Compared with the model group,the expression of PDGF-BB of each treatment group decreased significant(P＜0.01).the expression of PDGF-BB between each treatment group showed no significant difference(P＞0.05).Compared with the model group,the caspase-3 in olive of each treatment group decreased significant(P＜0.01 or P＜0.05). The caspase-3 of lower dose TCM group was lower significantly than the Clochicin group(P＜0.05).The caspase-3 of the higher Ganxinning dose and lower Ganxinning dose showed no significant difference(P＞0.05).Compared with the control group,the bcl-2 of each treatment group increased significant(P＜0.01).Compared with the model group,thebcl-2 between each treatment group showed no significant difference(P＞0.05).Conclusion:"Ganxinning"capsule can prevent HF of the experimental rats effectively and decrease the enzymology index of ALT,AST,TBA and the HF index of LN,HA,PⅢNP.The possible mechanism is that Ganxinning can depress the HSC multiplication which were transfected by PDGF and bFGF. The other possible mechanism is that it can adjust the caspase-3,bcl-2 to prevent the apoptosis of liver cell.The TCM was used on HF mostly through multi-approach,multi-hiberarchy and multi-target.All of these experiment data need to be verified by clinical study.