Electric Memory Hippocampus Signal Transduction Pathway Addiction For Heroin-dependent Mice

Objective:To study the effect of the Ca²⁺ signaling pathway and p-CREB in the hippocampus on experimental heroin psychological dependent mouse, and to reveal the potential neuromechanism of addictive memory by the low frequency electroacupuncture.To provide experimental evidence of preventing relapse with the acupounture.Method:Make the experimental heroin psychological dependente model with heroin conditioned place preference(CPP) in mice,48 mice were equally and randomly divided into 3 groups after pretesting:a control group,a model group and an electroacupuncture(EA) therapy group,needling the "Neiguan(PC6)" "Shanyinjiao(SP6)" "Sisheng chong" points.The behaviour index was measured by CPP(conditioned place preference),and the expression of CaM,CaMKâ…¡a,p—CREB in the hippocampus measured by RT-PCR and Western Blotting.Result:1.The mice of the model group stayed longer in the heroin-paired chamber than that of control group(P＜0.01);the therapy group stayed shorter than the model group.2.CaM and CaMKâ…¡a mRNA expression in hippocampus of model group increased compared with control group(P＜0.05); the expression of CaM and CAMKâ…¡a mRNA was decreased after EA therapy.3.Compared with control group,CaMKâ…¡a and p—CREB protein expression in hippocampus of model group mice rised significantly(P＜0.05), the expression of CaMKâ…¡a and p—CREB protein reduced in the therapy group(P＜0.05).Conclusion:Electric needling the points could partly quench the craving by heroin-related cues,possibly through to regulate the Ca^2- signaling pathway and the expression of p—CREB protein in the hippocampus of mice with psychological dependence on heroin,and to interfere in the process of addiction memory consolidated by EA therapy.