Yizhiren Salt Sunburn Reduction In Urine Role

Alpiniae Oxyphyllae(Alpinia Roxb,Zingiberaceae) is the dry ripe fruit of Alpinia oxyphylla Miq.with kernal as its medicinal part.The crude Alpiniae Oxyphyllae Fructus(CAOF) is pungent in flavour,warm and dry in property,acting on the pleen channel,and good at wanning the pleen and stopping diarrhea,astringing and preserving the droll-spittle,usually being used to treat abdominal pain,vomiting and diarrhoea,uncontrollable drolling.The stir-heated Alpiniae Oxyphyllae Fructus (SAOF) with salt soltuion can moderate the pungent,warm property,act on the kidney channel and the lower jiao exclusively,and specialize in conserving essence and arresting polyuria,usually being used for emissions,premature ejaculation, frequent mieturition and enuresis resulted from kidney qi deficiency cold.Aims:Being a branch of "technical commonness in the processing medthod of stir-heating with salt solution"under the State Ministry of Science and Technology 11th Five-Year Plan Support,our study aimed at the traditioanl medicinal herb Alpiniae OxyphyUae Fructus having affirmative clinical therapeutic effect,focused on the differences between the effects and clinical applications respectively before and after processing,expecting to elucidate the mechnism and active components for the arresting polyuria effect of SAOF,further interpreting the scientific connotation of the theory for "The stir-heated drug with salt solution acts on kidney,and the arresting polyuria effect",and then providing a new idea for the investigations on the drags processed by common techniques in stir-heating with salt solution.Methods:The differences in the arresting polyuria effect of Alpiniae Oxyphyllae Fructus before and after stir-heating with salt solution were focused by both of pharmacodynamical and chemical means.1.According to the present study situations of the animal models of frequent micturition and polyurine at home and abroad,the mouse metabolic cage was employed to perform empirical study of three types of animal models of frequent micturition and polyurine. 2.With the fixed herbal sources and fixed processing methods,the acute toxicity and the influences on the noraml and water-loading mouse of CAOF and SAOF were studied,and the crucial factors of SAOF for arresting polyuria were investigated.3.In the course of urine production,using water-loading mouse model of polyurine as the study object,the mechanism was investigated through the quantification of electrolyte,plasma aldosterone and plasma antidiuretic hormone;in the course of urine excretion,the mechnism was investigated on the effects on the ex vivo detrusor of bladder.4.Prelimianry tests were carried out to study the chemical compositions of CAOF and SAOF.The acitve fraction with the arresting polyuria effect of SAOF was screened and prepared,of which the acute toxicity and dose-effect relationship were studied.The initial investigations were carried out on the chemicial compositions of that fraction,such as the prelimianry tests,assaying of total terpenoid and G-C-MS analysis. The salt content limitation of SAOF was considered as well.Results:1.The metabolic cage suitable for the mouse urine collection was prepared,and the stable and controllable water-loading mouse model for polyurine was developed.2.The maximum dosages of CAOF and SAOF for the acute-term toxicity test were 924.4 times and 888.8 times of the clinical adult dosage,respectively.3.With 80 times adult dosage,both of them showed the ability to reduce the urinary volume of normal mouse significantly;for the water-loading mouse model of polyurine,the crucial working substance of SAOF was the drug itself,and the salt played a synergistic role.CAOF took effect at 120 times adult dosage,while SAOF at 80 times.4.CAOF significantly reduced the plasma aldosterone concentration of water-loading mouse and showed the effect of guaranteeing a sodium row of potassium,whereas this effect decreased after stir-heaing with salt solution;SAOF significantly increased the plasma aldosterone concentration of water-loading mouse; both of CAOF and SAOF stimulated the ex vivo bladder of Cavia procellus via acetylcholine antagonism with an increasing dosage,and SAOF showed a stronger effect. 5.The acitve components of SAOF for arresting polyuria were"petroleum benzine fraction + salt",namely active group.With LD₅₀ of 375 times adult dosage this group showed some neurotoxicity;the working dosage is 100 times adult daytime dosage.The limitation of total terpenoid was not less than 15.1%referenced to Nootkatone in petroleum benzine fraction,and to establish the chromatographic fingerprint.16 chemical compositions of petroleum benzine fraction were identified using GC-MS.The limitation of salt content was tentatively set as 1.5%-1.8% referenced to chioridion.Conclusions:The effect of arresting polyuria of Alpiniae Oxyphyllae Fructus before and after stir-heating with salt solution are evidently different.The working dosage of CAOF was 120 times adult dosage while that of SAOF was 80 times,which indicates the rationality of the clinical applications of SAOF in the treatment of frequent urination and polyurine,intiaUy proving the TCM theory for "Stir-heating drug with salt solution acts on kidney channel,and kidney governs water".The effect of arresting urtrination of Alpiniae Oxyphyllae Fructus after stir-heating with salt solution attributes to the synergistic actions of the drug and salt,with "petroleum benzine fraction of Alpiniae Oxyphyllae Fructus + salt"as the active components.The action mechnism lies in the increased water reabsorption due to the increased ntidiuretic hormon secretion and its influence on the M-receptor of bladder smooth muscle as M-receptor inhibitor thus decreasing the construction of bladder smooth muscle.Innovations:1.Elucidating the synergistic actions of Alpiniae OxyphyUae Fructus and salt for arresting polyuria initially,such can certify the theory about "the stir-heated drug with salt solution acts on kidney,and the arresting polyuria effect".2.Elucidating the active components and mechnisms for arresting polyuria initially.3.Developing the water-loading mouse model for polyurine,and applying for patent of utility model.