Neutralizing Response And Escape Mechanism Of Epidemic HIV-1 Infected Individuals In China

The protective immune response of HIV-1 infected individuals is still unclear.In China,the epidemic HIV strains included B'/C recombinant and subtype B' were different form those of Europe and Africa.We just study the neutralization respongse of HIV-1 infected individuals,and try to explain the escape mechanism from gp120 sequence.Plasmas from HIV-1 infected individuals in different epidemic area(Xinjiang Vygur Autonomous Region and Anhui province) were collected.Neutralization response of B'/C recombinant and subtype B'infected individuals detected by TZM-bl cell line;Binding antibody to SHIVchn19Env peptide of plasmas tested by ELISA. Coculture with negative PBMC to isolate clinical viruses;GHOST cell line analysis the coreceptor usage;gp120 sequences were expanded by RT-PCR and anlysised by bioedit software.Collusions:1.The neutralization level of plasmas from HIV-1 B'/C recombinant infected individuals were lower than that of plasmas form subtype B'infected individuals.2.The intrasubtype neutralization level of plasma was higher than intersubtype neutralization level.3.It has been found few plasma from HIV-1 infected individuals have higher and broadly cross neutralization level.4.Most HIV infected individuals could not neutralize the autologous isolates;however,there were few HIV infected individuals could neutralize autologous isolate with low level.5.There were no correlation between binding antibody and neutralization level of plasmas from HIV-1 infected individuals.6.There were negative correlation between neutralization sensitivity of HIV-1 isolates and neutralization level of plasmas from infected individuals.7.HIV-1 isolates could escape the neutralization pressure by adding N-glycans of gp 120 region.8.V3 structure determined the coreceptor usage.We first study the neutralization response of B'/C recombinant and B' subtype infected individuals.And try to explore the escape mechanism of isolates.This may help to develop the more effective AIDS vaccine.