Rheumatoid Arthritis Disease And Syndrome Treatment To Optimize The Experimental Study Of The Program And The Basic Side Paralysis More Soup

Rheumatoid arthritis(RA) is a chronic autoimmunity disease of which the main symptom is symmetry multiple arthritis.It is reported that the incidence rate of RA is 0.3～0.5%according to the rudiment epidemiological investigation in our country.The ratio of male to female is 1:3.RA regardless of for traditional Chinese medicine or Western medicine,is still a kind of difficult treat disease. Major is the cause of this disease with pathologyical mechanism lack all-sidely thorough understanding.Though it is reported that traditional Chinese medicine works well on it,but its research is restricted due to various classification standard of different signs of RA,and no unified standard and reproducibility of clinical effect.This research stresses on taking good advantage of seeking disease causes and determining treatment from patterns identified of TCM under the direction of Pro.Hu Yinqi,we choose the Demp-heat Obstruction Syndrome,the Cold-demp Obstruction Syndrome,the Difficiencecy of Silver and Kidney Syndrome,the Phlegm-blood Stasis Syndrome as an enter point to study the optimization project of RA treatment by TCM rules.The following is our research in three aspects:1 Literature study1.1 Advances in TCM of RAThe general situation of study on the TCM syndromes of RA and advances in TCM treatment of RA were reviewed on the basis of looking up a great amount of literature of RA.It was found that treatment of RA with TCM has the following advantages:good therapeutic effect and less side-effect etc.It was analysed the questions in the study on TCM syndromes and treatment of RA.The thoughts and methods of research in future were advanced.1.2 Advances in Western Medicine treatment of RAIn this part,we summarized the advances of treatment of Western Medicine,there wasn't an effective medicine be found with the exact effection till now,it make the treatment of RA in Western Medicine in a research situation.Most medicines had more side-effect than they worked,so we must search a point which can combine the treatments of RA in TCM and Western Medicine.1.3 RA and OPG/RANK/RANKL systemRheumatoid Arthritis is a course of bone erosion.The evidence coming from experiment shows that Osteoclast is necessary for this course.Osteoclast activation need the action of RANKL and it' s receptor RANK.As one of the nember of tumor necrosis factor(TNF),OPG can joint the RANKL by competitive combination,and stop the joint to RANK.It can inhibit the activation of Osteoclast and increase the apoptosis of Osteoclast to inhibit the absole of bone.1.4 Effect mechanism and side-effects of MTX in treating RAMTX,a kind of DMARDs,is widely accepted in treating RA for it' s confirmable effection,,high security.The text summarized the effect mechanism and side-effects of MTX in treating RA.1.5 The foundation of the research and the argumentation of feasibilityTo expatiate the foundation of the research and the argumentation of feasibility.2 Clinical study of optimization project in treating RA by TCM rulesObjective:This research stresses on taking good advantage of seeking disease causes and determining treatment from patterns identified of TCM and on the basis of the modern pharmacody study,combining the teacher' s clinical experience,we choose the Demp-heat Obstruction Syndrome,the Cold-demp Obstruction Syndrome,the Difficiencecy of Silver and Kidney Syndrome,the Phlegm-blood Stasis Syndrome as an enter point to study the optimization project of RA treatment by TCM rules.Method:All the patients were divided into four groups in random:the treatment group and the control group.The patients in the treatment group took BiYuTang(twice a day)and the patients of the other group took BiYuTang(twice a day) and MTX(10mg/w).Relevant criteria were then used to compare those groups in terms of general condition of the patients,clinical symptoms and signs and results from laboratory tests.All the data collected have been statistically analyzed with SPSS12.0 software.Results:Clinical research result shows,all the patients' clinical symptoms and signs and results from laboratory tests are effectively improved in the two groups(P＜0.05),the result has no obvious difference(P＞0.05),the side-effect of the treatment group is better than the control group(P＞0.05).The project of combination disease and syndrome to treat RA is effective.3 Trial researchObjective:In our studies we plan to further elucidate the mechanism of BiYuTang in curing bone erosion in RA by using the animal model of CIA rats,the CIA rats were feed with BiYuTang high dose,common dose and MTX for five weeks,HE staining sbserve pathological change under high microscope,detect the level of OPG and OPGL in serum.The aim of this study is to investigate the therapeutic mechanism of BiYuTang in curing bone erosion in RA through these methods.Method:50 health SD male mice was divided into 5 groups(normal group,model group,common dose group,high dose group and MTX group in random,each group contains 10 mice).In the first part,the CIA rats was successful established by injection the mixture of TypeⅡcollagen with Fraud' s adjuvants in ankle joint skin of both feet.After 1 week of immunization,the CIA rats were feed with BiYuTang high dose,common dose and MTX for five weeks except modle group.Common group and modle group were feed equal volume Sodium Chloride.During this period,the changes of the general status,swelling of the rats have been recorded at different time.After five weeks of treatment,the rats put to death and the serum was collected.HE staining observe the pathological change of ankle joint by microscope,ELISA to detect OPG,OPGL level in serum,the data was statistics by SPSS software.Results:BiYuTang high dose,common dose could markedly reduce the degree of rat pedal swelling,improve general status.The pathologicals results showed that synovialis hyperplasia and inflammation,inflammatory cell infiltration,formation of pannus and the erosion of cartilage and bones.Group of BiYuTang and MTX could lessen synovialis hyperplasia,restrain inflammatory cell infiltrate,inhibit pannus formation and relieve bone destruction.BiYuTang can increase the expression of OPG snd OPG/OPGL,decrease the osteoclast multiplication and activation,restrain or prolong the damage of bone.