Researches On Bone Densities Round The Screws And Discs Degeneration After Anterior Cervical Fusions

Objective.In the world,people have higher quality on life.On the same time, the aged will be the main stream and the traffic is more developed than any other years.So the spine injury,spine degeneration diseases and spine deformations occur more equent than before which need to be treat with internal fixation.We must know more situation about the spine after internal fixations to make the surgery method,instruments and the internal fixation technology better than before.Some research shows that the BMD is related to the power of screws extraction and the collapse of the implants.People also suggest that we should test the BMD of the cervical before fixing on them.Now the BMD exchangment around the screws has not been investigated.In these years,spine fusions have the higher achievement ratio which lead to the ASD occurring more often.The ASD of cervical spine after fusion is to show that osteophyma formation,narrow intervertebral space,vertebrae slippage, intervertebral disk hernia,spinal stenosis,flaval ligaments thickening and so on. ASD often occer on the upper segment of fusion and it has the rate of 6-60%after the cervical fusion.The TGF-β1 and the BMP-7 are researched as the treatment gene on intervertebral disk degeneration.Thompson described that the TGF-β1 can enhance the PG,which is the first describtion on gene treatment.BMP nearly is on all of the organs which contains the cyemology,auxanology and so on.After 1990,lots of tests showed that rhBMP-7 can enhance the PG of the rabbits. However AnHS got the contrast goal.Goat is the best animal as the model of anterior cervical fusions because it has the characteristic of rectal head and perpendicular weight loading.Besides,goat has the same size of intervertebral disk as person and the same biomechanics as people.The goat has been used to test the role of GF,bone graft,and so on.After anterior cervical fusion,the BMD,BMC are related to the screws extraction.BMD is an important index on end-plate mechanics.The test of centrum vertebrae BMD is significant to precede the implant sinking,screws extraction and false articulation.Intervertebral disk is the important structure in spine motion function. Retrogradation and injury are the main reasons to damage it,which can cause not only the intervertebral disk diseases,but also disturb the spine motion.Many researches showed the BMP-7's role on the biomechanics.Now,the research on BMP-7 is just on the rabbit about the injury and degeneration.Our test will establish the animal model on anterior cervical fusions and research the bone density around the screws,biochemistry in intervertebral disks and the imageology variations.All of above are served for clinic treatment by providing the evidence.Methods.1 body position:dorsal position,with the extremities binded on the operation table2 transfusion3 anaesthesia:general anaesthesia with tracheal intubation4 grouping:dividing into 3 groupsⅠ:normal 4 goatsⅡ:fixation 3 months 5 goatsⅢ:fixation 6 months 5 goats5 operation:6 post-operation:without external fixation,with antibiotics 5-7 days7 breeding:foodintake freely8 type of executing:asphyxiation9 segment of intervertebral disks:C2/3 and C5/610 position:nucleus gelatinosus annular fibrosus centrum vertebrae11 items of test:Immunohistochemistry BMD imageology PCRResults.After establishing the models and researching all the data,we get the results as follows:(1)Degenerations on intervertebral discs appear more earlier and serious than the control.(2) The bone density around the screws are decreasing more earlier and serious than the control.(3)The MRI shows that Degenerations on intervertebral discs appear more earlier and serious than the controlConclusion.Establishing the goat model of anterior cervical fusion and researching the bone density around the screws,biochemistry in intervertebral disks and the MRI variations,which show that ASD must have happened after operation.Our surgeons should consider it.