| Objective To develop a novel stent for colonic anastomosis and develop a new degradablematerial for this stent.Methods With the help of institute of polymer science of Zhejiang University, we synthesize thematerial with 1,3-propanediol, 1,2-propanediol, and sebacic acid, and designed the stent accordingto anatomic structure of colon and the need for anastomsis. Tensile rate, tensile strength, elasticmodulus, and elongation at break of the material were tested by pull machine.Results A new degradable high polymer material was developed, its molecular weightdistribution was 2.04, tensile rate was 500mm/min, tensile strength was 16.13Mpa, elasticmodulus was 24.73MPa, and elongation at break was 587%. A colonic anastomosis stent wasdesigned and produced.Conclusion A colonic anastomosis stent and a degradable material for it were developed. Objective To develop a novel technique of colonic anastomosis with degradable stent, which is characterized of simple procedure, fast colon anastomosis, and strong anastomotic stoma.Methods Colonic anastomosis with degradable stent was performed on 15 pigs, constituting stent anastomosis group (SA), and conventional hand-sewn colonic anatomosis was performed on 15 pigs, constituting conventional anastomosis group (CA). Stomas were observed on the day 3, 7, 14 postoperatively. Anastomosis time, adhesion score, bursting pressure, hydroxyproline content, and microvessel density were compared and the breaking strength of stent group was tested to evaluate the feasibility and safety of this technique.Results Each colonic anastomosis with stent was completed successfully. The anastomosis time of stent group was significant less than the control group (6.7±2.7 versus 14.8±2.7, P=0.000). No death, no stomal leakage or stenosis occurred in either group. The bursting pressure of SA was significant higher than CA on day 0, 3, 7 postoperatively. In SA, the breaking strength of each specimen was higher than 6.5N (half of the breaking of normal colon). There was no significant difference in hydroxyproline content, microvessel density, and adhesion score between the two groups.Conclusion The technique of colonic anastomosis with degradable stent is safe and feasible, it had less anastomosis time, and higher stomal strength compared with had-sewn technique. Objective To develop a safe and effective technique for primary repair of colon stomal leakage with the stent, it can complete primary repair safely even in severe abdominal infection.Methods Primary repair of colon stomal leakage with the stent was performed on 10 pigs, constituting stent repair for colonic stoma leakage group (SRL), and the group of conventional hand-sewn colonic anatomosis in part 2 was used as control group. The healing of stoma was observed on the day 7, 14 postoperatively. Bursting pressure, hydroxyproline content, microvessel density, and expression of metalloprotease mRNA were compared and breaking strength of SAS was tested to evaluate the feasibility and safety of this technique.Results Each primary repair of colonic stomal leakage with the stent was completed successfully. No death, no stomal leakage or stenosis occurred in either group. One pig had intestinal obstruction for a purulence node formed in colon wall. The hydroxyproline content of SRL was significant higher than control group on day 14 postoperatively, but equal on day 7. The breaking strength of each specimen in SRL was higher than 6.5N (half of the breaking of normal colon). There was no significant difference in microvessel density, bursting pressure, and relative expression of matrix metalloprotease mRNA between the two groups.Conclusion The technique of primary repair of colon perforation with degradable stent is feasible and safe. Objective To develop a safe and effective technique for primary repair of colonic perforation, it can safely complete primary repair of colonic perforation occurred 12 hours before or the perforation with severe abdominal infection.Methods Primary repair of colonic perforation with the stent was performed on 10 pigs, constituting stent repair for colonic perforation group (SRP), and the group of conventional hand-sewn colonic anatomosis in part 2 was used as control group. The healing of stoma was observed on the day 7, 14 postoperatively. Bursting pressure, hydroxyproline content, and microvessel density were compared and breaking strength of SRP was tested to evaluate the feasibility and safety of this technique.Results Each primary repair of colonic perforation with the stent was completed successfully. No death, no stomal leakage or stenosis occurred in either group. The breaking strength of each specimen in SAP was higher than 6.5N (half of the breaking of normal colon). There was no significant difference in bursting pressure, hydroxyproline content, and microvessel density between the two groups.Conclusion The technique of primary repair of colonic perforation with degradable stent is feasible and safe.
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