Mechanism Study Of Yuan-primary Points On Blood Pressure In Rats With Hypertension And Hypotension

Background: Mechanism study of meridian points is always the investigative emphasis and hot spot in the domain of acupuncture and moxibustion. How to apply multi-subject and the best advanced technology and method of modern medicine to investigate fundamental theory of traditional acupuncture and moxibustion, mechanism study of meridian points, and to provide emphatic theoretical evidences for clinical application is a question that needs to be solved urgently by experts in acupuncture and moxibustion.Mechanism study of meridian points includes the specificity and dual-directional regulation of acupoints. According to the mode that studying from proposing hypothesis through animal experiment to authenticating it through large scale clinical test, carrying out studies on the specificity and dual-directional regulation of acupoints, summarizing the effective regularity adjusted by acupuncturing, elucidating the effective regularity of acupoints and the mechanism of began, conducted, conformed and generated by acupuncturing signal, we will provide significant foundation for contemporary acupuncture and moxibustion.Objective: To investigate the specificity and dual-directional regulation of Yuan-primary points through observing the effect of Yuan-primary points and non-points on blood pressure in rats with hypertension and hypotension. This research provided the new theoretical basis for the domain of the mechanism study of acupoints of acupuncture in effecting blood pressure, as well as establishing a breakthrough for the further research on the mechanism of the effective regularity of acupoints.Methods: This research included two parts of hypertension and hypotension. Both of parts were assigned into twelve Yuan-primary points groups, non-points group, model group and normal group. Each group had 6 rats. Rats in Yuan-primary points groups and non-points group received acupuncture at corresponding position, Yuan-primary points and non-points selected both sides. The rats in model group and normal group were put in the fixer, but not received acupuncture. No. 30 and 1 cun needles were used to puncture perpendicularly. Uniform reinforcing and reducing methods were applied for 5 minutes. The treatment had been conducted successively for 7 days. All rats' blood pressure was detected 5 times in succession. Recording general average of BP. Statistical analysis was carried out using SPSS 13.0 for Windows. Results and analyses:1. Comparison of the specificity of Yuan-primary points in depressing BP: Comparing with non-points group and model group, the significant difference indicated that acupuncture can depress BP (P<0.05). In twelve Yuan-primary points groups, Yangchi, Taichong and Taixi groups' effect of depressurization was the best. Comparing with three groups, the difference was not statistically significant (P>0.05). The result indicated that effect of depressurization of Yangchi, Taichong and Taixi was similar. Taiyuan, Shenmen, Taibai, Hegu and Wangu groups' effect of depressurization was better than non-points group's. Comparing with five groups, the difference was not statistically significant (P>0.05). The result indicated that effect of depressurization of Taiyuan, Shenmen, Taibai, Hegu and Wangu was similar. Comparing with Daling, Qiuxu, Chongyang groups and model group, the difference was not statistically significant (P>0.05). As well as comparing with Daling, Qiuxu, Chongyang groups and Jinggu, non-points group, the difference was not statistically significant too (P>0.05). These results indicated that Daling, Qiuxu, Chongyang can depress BP and its' effect was similar.2. Comparison of the specificity of Yuan-primary points in boosting BP: Comparing with non-points group, twelve Yuan-primary points groups and model group, the significant difference indicated that acupuncture can boost BP (P<0.05). In twelve Yuan-primary points groups, Shenmen, Daling, Hegu and Yangchi groups' effect of boosting BP was the best. Comparing with four groups, the difference was not statistically significant (P>0.05). The result indicated that effect of boosting BP of Shenmen, Daling, Hegu and Yangchi was similar. Taiyuan, Taichong groups' effect of boosting BP was not as good as Shenmen, Daling, Hegu and Yangchi groups' . Comparing with two groups, the difference was not statistically significant (P>0. 05). The result indicated that effect of boosting BP of Taiyuan, Taichong was similar. Wangu, Taixi, Taibai groups' effect of boosting BP was not as good as Taiyuan, Taichong groups' . Comparing with three groups, the difference was not statistically significant (P>0. 05). The result indicated that effect of boosting BP of Wangu, Taixi, Taibai was similar. Jinggu, Chongyang and Qiuxu groups' effect of boosting BP was the worst in twelve Yuan-primary points groups, but was better than non-points group. Comparing with Jinggu, Chongyang and Qiuxu groups and non-points group, the difference was significant (P<0. 05).3. Comparison of the dual-directional regulation of Yuan-primary points in regulating BP: Comparing with twelve Yuan-primary points groups, model group and non-points group, the significant difference indicated that its' dual-directional regulation in regulating BP was different (P<0. 01). Twelve Yuan-primary points groups' dual-directional regulation in regulating BP was better than model and non-points groups' . In twelve Yuan-primary points groups, Hegu, Yangchi and Shenmen groups' dual-directional regulation was the best. Comparing with three groups, the difference was not statistically significant (P>0.05). The result indicated that the dual-directional regulation of Hegu, Yangchi and Shenmen was similar. Taiyuan, Taichong and Wangu groups' dual-directional regulation was not as good as Hegu, Yangchi and Shenmen groups' . Comparing with three groups, the difference was not statistically significant (P>0.05). The result indicated that the dual-directional regulation of Taiyuan, Taichong and Wangu was similar. Daling, Taixi, Taibai groups' dual-directional regulation was not as good as Taiyuan, Taichong and Wangu groups' . Comparing with three groups, the difference was not statistically significant (P>0.05). The result indicated that the dual-directional regulation of Daling, Taixi and Taibai was similar. Chongyang, Jinggu and Qiuxu groups' dual-directional regulation was the worst in twelve Yuan-primary points groups. Comparing with Chongyang, Jinggu, Qiuxu and non-points group, the difference was not statistically significant (P>0. 05). The result indicated that its' dual-directional regulation in regulating BP was similar.Conclusions:1. Acupuncture can depress BP. And its immediate effect of depressing BP was significant, but persistence effect of depressing BP need be authenticated by large scale clinical test. The specificity of Yuan-primary points in depressing BP was different. In twelve Yuan-primary points groups, Yangchi, Taichong and Taixi's effect of depressurization was the best; Taiyuan, Shenmen, Taibai, Hegu and Wangu's effect of depressurization was not as good as Yangchi, Taichong and Taixi's; Daling, Qiuxu, Chongyang's effect of depressurization was the worst, and was similar to non-points'.2. Acupuncture can boost BP. And its effect of boosting BP was more steady and persistence than effect of depressing BP. The specificity of Yuan-primary points in boosting BP was different. In twelve Yuan-primary points groups, Shenmen, Daling, Hegu and Yangchi's effect of boosting BP was the best; Taiyuan, Taichong's effect of boosting BP was not as good as Shenmen, Daling, Hegu and Yangchi's; Wangu, Taixi, Taibai's effect of boosting BP was not as good as Taiyuan, Taichong's; Jinggu, Chongyang and Qiuxu' s effect of boosting BP was the worst, but was better than non-points group.3. Acupuncture on non-points and twelve Yuan-primary points in regulating BP has dual-directional regulation. The dual-directional regulation of Yuan-primary points in regulating BP was different. In twelve Yuan-primary points groups, the dual-directional regulation of Hegu, Yangchi and Shenmen was the best; the dual-directional regulation of Taiyuan, Taichong and Wangu was not as good as Hegu, Yangchi and Shenmen; the dual-directional regulation of Daling, Taixi, Taibai was not as good as Taiyuan, Taichong and Wangu; the dual-directional regulation of Chongyang, Jinggu and Qiuxu was the worst.