| Objective:Benign prostatic hyperplasia(BPH)is the common,frequently occurring disease of aging male.While the aging population increasing gradually,the incidence rate of BPH is ascending too,BPH has already affect their health and quality of life.It is uncertain about the etiopathogenesis of BPH,according to the data,it indicates that the reason results in hyperplasy of prostate interstitial substance around the urethra and glandular organ.As far as apoptosis concerned,because of over-hyperplasy or apoptosis disorder(programmed cell death),leads to the hyperplasy of cell,so,the theory of apoptosis has been paid more and more attention,it is considered that the apoptosis reduction and the cell multiplication participate the generative course of BPH,so how to maintain the stabilization of prostatic internal environment and interfere the progression of BPH,becomes the important aim of drug treatment.The research includes both the clinic and the experiment research,through the method of immunohistochemistry,probes the mechanism and the connection of traditional Chinese medicine YinShenTong capsule(YST capsule)in curing BPH,as well as the effection in the cell multiplication and apotosis of human and rat,so as to provide the data for treating the BPH with traditional Chinese method.Content1.Clinical resarch(1)Material and methodFollowed the principle of randomization,comparison,according to 1:1 proportion,numbers the internalized standard target by order and divided them into the YiSTtreatment capsule group(The YST capsule group)and the finasteride control group(the finasteride group).The YST capsule group was taking orally with YST capsules for 3 courses of treatment,and the the finasteride group was taking orally with finasteride tablet for 3 courses of treatment.After 3 courses of treatmet,operation of BPH treatment were accepted and the patients prostate tissue taken out.for the sake of comparison,an extra normal group was added up just for comparison.By means of immunhistochmisty,determine the expression of bcl-2/bax,Ki-67/CD34 in the 3 groups.Through the expression,observed the information of prostatic apoptosis,and evaluated the influence of YST capsule to human prostatic apoptosis.(2)Result①The bcl-2 protein expression of prostate hyperplasia tissue is obvious higher than the normal prostate tissue(P＜0.01),It indexed that the over-expression of bcl-2 protein induced the apoptosis to decrease,and played an important part during the developing process of BPH.bcl-2 protein expression of prostatic hyperplasia tissue is obvious higher than the normal prostate tissue,but the apoptosis is on the contrary,the result is accord to the report before,so there are inverse correlation of the bcl-2 protein expression and the apoptosis②The bax protein expression appeared in both protstate endothelial cell and interstitium,especially expressed obviously in endothelial cell.Though the bax protein expression was lower in hyperplasia prostate tissue than in the normal prostate tissue,but there was no statistical significance(P＞0.05), it meant that bax protein expression played an unimportant part in the generative,developing procession of BPH.③Bcl-2 protein expression was no obvious difference in both treatment group and control group(P＞0.05),the positive expression rate of bax protein makes no obviously different in both 2 groups(P＞0.05),It proved that the mechanism of curing BPH with YST capsule was promoting prostate hyperplasia apoptosis, so as to diminish the volume of prostate,relieve the oppressive and irritative symptom of BPH,the therapeutic efficacy was similar to finasteride tablet.④Both the YST capsule and the finasteride tablet could lower the expression of Ki-67 and CD34,it proved to be restraining the new vessel of prostate as to cure BPH,and believe that was the mechanism of YST capsule and the finasteride tablet. 2.experiment research(1)Material and method75 Sprague-Dawley male rats were randomly divided into 5 groups.Included normal group,hyperplasia group,YST capsule large-dosage group,YST capsule low-dosage group,finasteride group.When experiment models were finished, except the normal group,the rest of groups were given intragastric administration with different drugs.The rats were killed and prostate were removed after intragastric administration for 30 days.By means of immunhistochmisty,determine the expression of bcl-2/bax,Ki-67,to evaluate the influence of YST capsule to rat prostatic apoptosis.(2)Results①Compared with hyperplasia group,the weight,volume and index of prostate in YST capsule large-dosage group,YST capsule low-dosage group,finasteride group were obviously less(P＜0.05 or P＜0.01);Compared with finasteride group, although the weight,volume and index of prostate in YST capsule large-dosage group,YST capsule low-dosage group werehigher,but there were no obviously difference.The research proved to that the effect of YST capsule large-dosage, YST capsule low-dosage is similar to finasteride group,also could lower the weigh,volume,index of rats prostates.②Through the observation of rats prostate tissue HE corlored,it proved that the YST capsule large-dosage,YST capsule low-dosage could improve the tissue pathohyperplasia of rats prostates.③Compared with normal group,the bacl-2 protein expression of YST capsule large-dosage group and YST capsule low-dosage group are much higher,there are significant different(P＜0.01).Compared with hyperplasia group,the bacl-2 protein expression of YST capsule large-dosage group,YST capsule low-dosage group and finasteride group is lower,it make obviously different (P＜0.05);the bacl-2 protein expression makes no obviously different(P＞0.05) of the YST capsule large-dosage group and YST capsule low-dosage group to be compared with finasteride group;the positive expression rate of bax protein makes no significant different among the 5 groups.It proved that the YST capsule can balance the expression of bcl-2 and bax protein and promoted the prostatic apoptosis.④Ki-67 protein did not express in the prostate tissue of normal group. Compared with normal group,the expression of Ki-67 protein in the hyperplasia group,the YST capsule large-dosage group,YST capsule low-dosage group and the finasteride group was higher(P＜0.01),there was significant difference. Compared with the hyperplasia group,the expression of Ki-67 protein in YST capsule large-dosage group,YST capsule low-dosage group was lower obviously (P＜0.05 or P＜0.01).Compared with the finasteride group,the expression of Ki-67 protein in YST capsule large-dosage group and YST capsule low-dosage group was high,among them,there was statistical significance that the expression of Ki-67 protein increased in YST capsule low-dosage group(P＜0.05). there was no statistical significance that the expression of Ki-67 protein increased in YST capsule large-dosage group(P＞0.05).it was no significant difference between YST capsule large-dosage and YST capsule low-dosage,it was proved that both of them can lower the expression of Ki-67 of rat prostate tissue,restrain the prostate cell multiplication,promote prostatic apoptosis.Conclution①the pathogenesis of this diseases is intermingled deficiency and excess. root cause is deficient and the manifestation is excess,deficiency of kidney qi is the main reason,and the blood stasis is the main part of Pathological Mechanism,and survives all through the process of BPH,so,the Chinese medicine which invigorate kidney qi and promote blood circulation for removing blood stasis,is more and more used in curing BPH,and show their important effect.②The research are engaged in the method of apoptosis according the former clinic and experience research result.It is poly-link and multitarget for the YST capsule to cure BPH,it can lower the weigh,volume,index of rats prostates;it can improve the tissue pathohyperplasia of rats prostates; It can balance the expression of bcl-2 and bax protein;it can lower the expression of Ki-67 of rat prostate tissue,restrain the prostate cell multiplication,promote prostatic apoptosis.③It proves that the mechanism of curing BPH with YST capsule is promoting prostate hyperplasia apoptosis,restraining the new vessel of prostate, lowering the cell activity of cell multiplication,so as to prevent the BPH from happening,and its mechanism of action is synthetic.It is a variety of safe and effective Chinese medicine,and get a great application prospect. Butall the research above is partly and preliminary,it is expected to be deeply approached in the future. |
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