Changes In The Endogenous Cannabinoid Receptor In Fat In Obese Rats Cardiovascular System And The Endogenous Cannabinoid-like Substance Anandamide Outward Potassium Current In Cardiac Myocytes

PART I Changes of endocannabinoid receptorsin the myocardium and aorta of high-fat-diet-induced obese ratsObjective: To compare the cannabinoid receptor1 ( CB1 ) 's andcannabinoid receptor2(CB2)'s level in the hearts and aorta ofhigh-fat-diet-induced obese rats(HFD rats) and regular diet control rats (RDC rats) .Methods: We used high-fat-diet to induce obesity in male Wistar rats. After twenty weeks, the mRNA of CB1 and CB2 in the myocardium and aorta of HFD rats and RDC rats were tested by means of Real Time Polymerase Chain Reactions. And we also used Western Blotting and immunohitochemistry tests to learn the protein level of CB1 and CB2 in the myocardium and aorta of HFD rats and RDC rats.Results: The mRNA of CB1 and CB2 in the myocardium of HFD rats are significantly down-regulated (respectively 1:74.23 and 1:128.80 compared with RDC rats, P <0.05) . The CB2 mRNA in the aorta of HFD rats are also significantly down-regulated (1:21.17 compared with RDC rats, P <0.05). The CB1 protein in the aorta of HFD rats are significantly increased compared with RDC rats(respective IOD values are 5112.75±900.66 and 3317.89± 1244.00, P<0.05). The myocardial CB1 protein and CB2 protein of HFD rats and RDC rats are not significantly different according to Western Blotting.Conclusion: The myocardial CB1 and CB2 receptors are down-regulated in...