Injection Studies Of Toad Venom Delivery System Of Solid Lipid Nanoparticles

The whole body chemotherapy and hormone therapy may be the good means to cure some tumors. Being the assistance treatment of the operation and radiation, the whole body treatment is also an important treatment method. But the chemotherapy (include to use the chemistry medicine, Traditional Chinese Medicine and natural medicines) exists some bad reactions including the toxicity problems. The anti- tumor product which name is Chansu injection used on the clinic extensively also has the bad reaction of vascular irritation, and its anti-tumor function not obvious. In order to resolve this problem, designed to take the solid lipid nanoparticles as the drug's carrier, hoping to decrease the blood vessel irritation and enhance drug distribution in the tumor.The safety evaluation of the solid lipid nanoparticles (SLN) which was mainly made of Glyceryl behenate (ATO) had been made to inquiry the possibility that it fitted for intravenous administration. The results indicated that ATO was safe, effective and controllable as the carrier of drug for intravenous administration.The determination method of cinobufagin and resibufogenin in Chansu was established, and the main physical and chemical property of Chansu extraction (Cs) like partition coefficients (ratio cinobufagin and resibufogenin in lipid to the amount of cinobufagin and resibufogenin in aqueous phase) was studied. Cs-SLN was prepared by high shear homogenization, high pressure homogenization (hot-high pressure homogenization, cold-high pressure homogenization) and ultrasonication. Cs-SLN was evaluated by its mean size and entrapment efficiency. The partition coefficients obtained was 146.1 ± 0.56(ml/ g), cinobufagin and resibufogenin were highly soluble in lipid; ATO was selected as preparation material of Cs-SLN. Cold-high pressure homogenization was selected for preparation method of Cs-SLN.Cs-SLN was prepared by cold homogenization technique. The effects of influence factors such as the weight of ATO, soybean lecithin and poloxamer 188, on mean size, entrapment efficiency, and drug loading and overall desirability were investigated by using central composite design and response surface method. The data were imitated...