The Injection Of Solid Lipid Nanoparticles Of Cucurbitacin

Solid lipid nanoparticles (SLN) is a promising drug delivery system with solid lipid as drug carriers , diameters ranging between 50 and 1000 nanometers, which has the advantages of high physical stability, slow speed of drug leakage and low toxicity etc. The objective of this thesis was to study cucurbitacin-loaded solid lipid nanoparticles (SLN) for intravenous administrationFirst, an HPLC method with acetonitrile-0.1mol/L K₂HPO₄(50/50,v/v) as mobile phase was developed for the assay of cucurbitacin B in drug and the formulation.According to the results of the study before formulation designing, emulsification-ultrasonic method was developed to prepare cucurbitacin SLN suspension, with stearic acid glyceride as lipid carrier. The optimal formulation was obtained by orthogonal experiment design. Moreover, a freeze-drying method was investigated in order to improve the stability of the cucurbitacin SLN suspension.The quality of the cucurbitacin SLN suspension was evaluated before and after freeze-drying. The results showed that the cucurbitacin SLN suspension prepared in this thesis were regular and well distributed with a mean diameter of 128±18nm, a zeta potential of -46.0mv, an encapsulation efficiency up to 71.6%, a drug-loaded capacity of 3.72% and a pH close to 6.0. However, the freeze-dried SLN showed a mean diameter of 150±34nm, a zeta potential of -30.8mv, an encapsulation efficiency of 72.9%, a drug-loaded capacity of 3.72% and an unchanged pH. Results of the stability experiments indicated that the cucurbitacin SLN suspension could be kept stable at 4℃ for one month only,...