Genitourinary Tract Chlamydia Trachomatis Infection In Serology And Molecular Biology Research

Urogenital Chlamydia trachomcitis infectious, the common sexually transmitted disease in China, can progress to chronic inflammatory diseases that are associated with serious long tenn sequelae such as ectopic pregnancy and tubal infertility. There have been suggestions that chlamydial antigens of 57-60 kD may be involved in the immunopathology. Two important 57-60 kD antigens are a Triton X-100-soluable antigen, which involved in hypersensitivity in ocular models, and a Triton X-100-insoluable cysteine-rich structural protein, omp2. The Triton X-100-soluable antigen, believed to be a member of the family of widely conserved 60 kD heat shock proteins (HSP60), has been implicated as a major target antigen that stimulates the immunopathological response.A series of studies were conducted winch focused on human serologic reaction to different C. trachomatis antigens, including HSP60, after genitourinary chlamydial infections, genotyping of C. trachomatis isolates and its association with clinical manifestations and serologic response, amplification and cloning of gene encoding HSP60.1. By application of cell culture for C. trachomatis, immunoblotting and micro immunofluorescence assay(MIF), three patient groups, non-gonococcal urediritis (cervicitis) (NGU), pelvic inflammatory disease (PID), and infertility, and a healthy control group were evaluated. Urogenital C. trachomatis infections were common in NGU and PID groups and less often...