Fibronectin And Pulp Cell Differentiation, The Pulp Tissue To Repair The Relationship

The extracelluar matrix(ECM) is main part of the dental pulp tissue, it constitutes the enviroment where the dental pulp cells live and metabolise. The character of the dental pulp tissue is determined by ECM in one side. The interaction of cells with ECM is important for the regulation growth, migration and differentiation of cells.Fibronectin(FN) is one of the major glycoproteins of ECM, it has relatively widespread distribution in the dental pulp tissues, and might play a role in cell differentiation and fuction, acts as a mediator of cell adhesion both to other cells and to extracellular components, the biological role of FN during development and repair has been well-characterized.The human dental pulp flbroblast-like cell is one of the major type cells in the human dental pulp tissue, it could be induced to differentiate into odontoblast-like cells by some inductive factors, and form the mineralized matrix such as dentine. Aim of this study was to evaluate the effect of FN on the differentiation or function of the dental pulp cells.(1) Localization of FN in or beside the odontohlast layer - in early human odontogenesis, normal and carious (shallow,middle) human permanent third molars.FN was to be identified by immunohistochemistry using specific antibodies and ABC-peroxidase labelling. First in the teeth germs, at the level of section where no signs of odontoblast were evident, intense reaction was observed in the cusp region where the cells codensed. At a later stage, when some ECM had been deposited next to basement membrane to form the predentine and dentine, the reaction was observed at the border zone between the distal end of odontoblast and predentine, along the odontoblast processes, the cytoplasm of odontoblast or between the odontoblasts. FN was enriched in the dental basement membrane at the time of odontoblast differentiation, moderate staining was observed in the...