Type ¢ñ Hypersensitivity Role In The Pathogenesis Of Hepatitis B

Chapter 1 Study on Correlation between type I hypersensitivity and acute hepatitis BObjective To investigate the pathogenesis of hepatitis B related with type I hypersensitivity by studying the immunological markers in the sera of patients with hepatitis B. Methods Enzyme-linked immunosorbent assay (ELISA), immunofruerecence technique, and radioimmunoassay (RIA) were used to determine serum levers of IgE, histamine, and plasma level of cAMP in the patients with acute hepatitis B respectively. Indirect non-specific mast cell degranulation and indirect HBsAg-specific mast cell degranulation were also tested. The combined anti-allergic treatment was used to treat patients with acute hepatitis B based on the results, and the therapeutic effectiveness was observed by examining patients' serum IgE and histamine levers, as well as clinical improvement. Results The total serum IgE and histamine levers, the percentage of mast cell degranulation, and the percentage of HBsAg-specific mast cell degranulation in the patients with acute hepatitis B were found to be significantly higher than those in the normal subjects, whereas, the plasma cAMP level was lower in the patient group. It was also observed that the therapeutic effectiveness in the combined anti-allergic treatment group was better than that in the control group, furthermore, the serum levers of IgE and histamine decreased after the treatment in the treatment group. Conclusion Type I hypersensitivity may play a role in the pathogenesis of acute hepatitis B.Chapter 2 Determination of serum IgE and IL-4 in patients with hepatitis BObjective To investigate the role of type I hypersensitivity in the pathogenesis of hepatitis B. Methods The IgE and IL-4 in sera from 60 patients with various types of hepatitis B and 20 normal subjects were determined using sandwich ELISA. The correlation of levels of serum IgE and IL-4 with serum alanine aminotransferase (ALT) were analyzed. Results The levels of serum IgE and IL-4 in various types of hepatitis B were higher than those in control subjects. Furthermore, the higher levels of serum IgE and IL-4 were also observed in the same patients with higher levels of ALT. Conclusion The type I hypersensitivity may play a role in the pathogenesis of hepatitis B.Chapter 3 Serum level of soluble intercellular adhesion molecule-1...