| Objective To observe the effects of the softening and resolving hard mass drugs in Huayusanjie tablet on hepatocyte growth factor(HGF), connective tissue growth factor(CTGE), hyaluronic acid(HA) in rabbit vitreous body of experimental proiiferative vitreoretinopathy(PVR) model, for exploring the mechanism of the softening and resolving hard mass drugs to prevent PVR. Methods Retinal pigment epithelium cells(RPEs) cultured in vitro were injected into rabbit vitreous body cavities respectively to establish experimental PVR model. Huayusanjie tablet or the softening and resolving hard mass drugs were fed respectively up to 60 days after the models had been established for 3 days. Then, RIA method and immunohistochemical method were used to detect the expression of HGF, CTGF and HA in vitreous body. Results 1; Huayusanjie tablet could significantly depress the compression of HGF and CTGF on proiiferative membrane, so did the softening and resolving hard mass drugs(P<0.01 or P<0.05) and the action of Huayusanjie tablet was more strong(P<0.05). 2. Compared with the model control group, there was significant lower HA content in the Huayusanjie tablet group or the softening and resolving hard mass drugs group(P<0.01); and compared with the softening and resolving hard mass drugs group, there was significant lower HA content in the Huayusanjie tablet group too(P<0.05); 3. Compared with the low dosage group, the action of preventing PVR was more strong in the high dosage group of Huayusanjie tablet or the softening and resolving hard mass drugs respectively. Occlusion There is certain relation between the... |
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