Primary Study On Efficiency And Mechanisms Of Peimine Reversina Multi-drus: Resistance Of A549/DDP Cell Line

Objective:1.To study theory of Traditional Chinese Medicine(TCM)between sputum evil andLung Cancer multi-drug resistance(MDR).2.To explore correlation between syndromes of TCM and effect of chemotherapyabout Lung Cancer.3.To explore effect and mechanism of MDR in adenocarcinoma line A549/DDPreversed by peimine.Methods:1. Through reviewing literature of TCM about Lung Cancer, contrasting featuresof sputum evil and Lung Cancer MDR,dissertation put forward a hypothesis.2.On base of clinical practice,the dissertation supported hypothesis byretrospective analysis in282Lung Cancer patients.3.On base of theoretical and clinical pratice,the dissertation developed experimentto assure the hepothesis.MTT assay was employed to measure cell resistant index andreversing effect, flow cytometry was used to analyse apoptosis, cell immunofluorescencetechnique was used to measure expression of resistance associated protein LRP, RT-PCRwas employed to evaluate expression of ERCC1-mRNA.Results:1.The dissertation put forward a hypothesis that sputum evil might be importantreason of MDR,Huatan Sanjie might be basic law of reversing Lung Cancer MDR.2.The clinical study showed that RR,TTP,OS in team of Vital qi deficiency bemuch better than in the other groups. 3.Resistant index in A549/DDP line was13.08.There were common feature that cellinhibitory rate increased with the increase of DDP concertration among blank contronlgroup,DDP contronl group,Tetramethylpyrazine experiment group and peimine experimentgroup.Reverse index of Tetramethylpyrazine experiment group was3.62,thus that ofpeimine experiment group was3.73.Apoptosis rate of the four group was respectively2.56±0.44,13.5±1.42,33.82±4.56,38.16±2.25,in which that of the experiment group wasmuch more than that of the control group.Cell immunofluorescence technique showed thatthe expression of L-RP in experiment group was much lower than that in the control group(p <0.05).The expression of ERCC1mRNAwas respectively0.62±0.04,0.56±0.03,0.36±0.07,0.32±0.08,the difference between the experiment group and the control groupwas statistically significant(p <0.05).Conclusions:1.On the base of the literatural study and analogy,the doctoral dissertation putforward a hypothesis,and provided it by lots of evidence.2.Vital qi deficiency was the most better prognosis of all syndromes. RR,TTP,OSwould decrease when sputum evil,blood stasis,heat evil appeared.The phenomenonwould be the result of MDR.3.Peimine could reverse MDR in A549/DDP cell line.Inducing apoptosis, down-regulating L-RP and ERCC1mRNA express were the possible mechanisms.