| ObjectiveLung cancer was the most common malignant tumor of clinical, the incidence rate of cancer mortality in the first rank. Professor Qiu Xingfan mentor with many years experience in clinical treatment of lung cancer proposed the "dirty end virtual network paralysis virus" as the main pathogenesis of lung cancer, especially "cancer drug knot together" was the key to the incidence, clinical drug commonly used method of attack insects, vegetation detoxification method elimination of toxin, proved toxic method of attack insects, vegetation, detoxification method was effective, as the basis for this study, combined with modern medical research, in-depth study of lung cancer etiology, pathogenesis, and the experimental study drug law and observing insects attack vegetation Toxin on C57BL/6 mice with Lewis lung cancer xenograft model differences in the role of lung cancer, the survival status of mice by observing the subjects, body weight, thymus weight and thymus index of toxic side attack of insects, vegetation, toxic side immune function of mice tested; by detecting the inhibition rate of Lewis lung carcinoma mouse tumor cell proliferation index and morphology, drug side attack of insects, vegetation, toxic side effects of lung cancer prevention and control mechanism, and worms, virus attack detoxification side and vegetation between the two groups to improve immune function, inhibit tumor cell proliferation, the role of the comparison.The insects attack drug detoxification for vegetation Tongluo strong side effect, so by observing the tumor vascular morphology and vascular endothelial growth factor (VEGF-C) expression of virus attack insects and vegetation Toxin Act similarities and differences between the mechanism of anti-lung cancer. At the same time by detecting the mouse Lewis lung carcinoma tumor suppressor gene P53 protein expression and P53mRNA expression of oncogene c-myc protein expression and the expression of c-myc mRNA, and explore the toxic side attacking insects, vegetation, prevention and treatment of lung cancer may be toxic side mechanism, and the insects attacking side and the vegetation detoxification drug side P53, c-myc expression in comparison. Investigate the mechanism for the drug attack insects in the medical method, vegetation detoxification methods for the treatment of lung cancer provide the basis of experimental data and applications.Method1.The theoretical study, literature review, clinical practice, the method produced by lung cancer etiology, pathogenesis and drug characteristics for the cancer drug, respectively, and detoxification methods to attack a detailed discussion of modern medicine and our clinical research work experience in the treatment of the disease, poison attack insects and vegetation proposed antidote in the treatment of lung cancer treatment process plays a different role in both, in which the role of insects attack more poison Jun strong collaterals. When combined with clinical patients with different physical and cameras while the application of different courses.2.There were 5 groups in the experiment:control group, model control group, drug group attacking insects, vegetation detoxification group, cyclophosphamide group.By the recovery, vaccination, conservation Lewis lung tumor lines, select a good growth of Lewis lung cancer, cut and homogenized into cells were inoculated subcutaneously in each mouse in the right armpit. Copy C57BL/6 mice Lewis lung cancer xenograft model. Selected based on clinical experience instructors centipede, gecko, dew worm attack drug side honeycomb form, Solanum nigrum, white British, white composition of vegetation detoxification square off fleas. Model control group, drug group attacking insects, vegetation, the day after detoxification group received oral administration for 12 days, intraperitoneal injection of cyclophosphamide. On day 13 mice were sacrificed by cervical dislocation. To take samples, make the following test:the normal control group, model control group, drug group attacking insects, vegetation detoxification group, cyclophosphamide group were living condition, changes in body weight, thymus weight, thymus index, tumor inhibition rate of tumor cell cycle, tumor pathology light microscope and electron microscope morphology; RT-PCR method to observe the model control group, drug group attacking insects, vegetation detoxification group, cyclophosphamide group were tumor cells VEGF mRNA, P53 mRNA and the expression of c-myc mRNA situation; by immunohistochemical detection model control group, drug group attacking insects, vegetation detoxification group, cyclophosphamide group VEGF protein in tumor cells in mice, P53 protein and c-myc protein expression situation.Results1.The insects attack drug side vegetation detoxification only significantly reduce tumor cell proliferation index, but the effect was weaker than cyclophosphamide group; 2.Drug party worm attack, vegetation, toxic side effects on the thymus of cyclophosphamide inhibition of less than group, and can increase the thymus index, which play a role in enhancing immune; 3.Tumor weight in mice with tumor suppression, the drug party worm attack, the plants were toxic side can reach 45.96%,26.68%, worms attack toxic side effects than inhibition of tumor detoxification side vegetation; 4.In terms of improving the quality of life, excessive direct impact on tumor weight in mice quality of life, making the mouse has difficulties, failure to make traditional Chinese medicine to improve the quality of life in mice The index determination is uncertain; 5.In the morphology, the electron microscope shows toxic side attacking insects, vegetation, can only lead to tumor cell detoxification swelling of mitochondria, lysosomes and lipid bodies increase in nucleolus disappear chromatin margination at the nuclear membrane, the chromatin condensation, etc, worms attacking side than vegetation detoxification drug side; HE staining and observed under light microscope, showing that drug groups attack insects, vegetation, detoxification group was significantly the number of tumor microvessel less than the model control group and the cyclophosphamide group, and the insects attack the number of drug group was significantly less than the Microvascular vegetation detoxification group; 6.Drug party worm attack, the plants can inhibit the toxic side expression of VEGF-C role, suggesting that inhibition of VEGF-C expression levels may be toxic side attacking insects, vegetation, toxic side one of the mechanisms of lung cancer prevention. Lewis lung carcinoma in mice aspects of tumor angiogenesis drug party worm attack detoxification side was better than grass, suggesting that we attack drug party worm Lewis lung carcinoma on the inhibition rate than detoxification side vegetation, the possible mechanism was due to drug party worm attack Lewis lung carcinoma inhibited VEGF-C expression was better than that of vegetation Jiedu; 7.Drug side attacking insects, vegetation, toxic side has increased the level of P53 expression, inhibit the expression of c-myc effect, suggesting improve the level of P53 expression, inhibit the expression of c-myc may be drug side attacking insects, vegetation, toxic side one of the mechanisms of lung cancer prevention. ConclusionDrug party worm attack, the anti-side vegetation detoxification Lewis lung carcinoma in mice the exact role and drug party worm attack rate in the tumor than detoxification side vegetation, which both parties can improve the level of P53 expression, inhibition of c-myc expression. Insects attacking anti-cancer drug side effects side detoxification better than grass, the mechanism may be due to toxic side worm attack Lewis lung carcinoma inhibited VEGF-C expression was better than grass detoxification side, which confirms the mentor Professor Qiu Xingfan's "network Bi" was an important pathogenesis of lung cancer point of view. Insects attacking drug side effects side detoxification mechanisms of vegetation mainly:1. Control tumor cell Go /S locus, the tumor cells were arrested in Go phase, no entry into mitosis, and thus inhibit tumor effect; 2.Worms attack drug class side, the vegetation on the Lewis lung cancer model detoxification side thymus weaker than the inhibitory effect of cyclophosphamide, thymic function can be activated, by regulating the body's immune system, promote the increase in the number of lymphocytes in tumor tissue, so as to achieve enhanced immunity effect; 3.Drug side attacking insects, vegetation, toxic side has increased the level of P53 expression, inhibition of VEGF-C, c-myc expression level of the role, thereby inhibiting tumor angiogenesis, tumor cell proliferation; 4.This study reveals a worm attack drug class side, the vegetation part of the mechanism of detoxification side, but the role of traditional Chinese medicine research in multi-target and the objective existence of complex, suggesting that we should further thinking and further study.
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