The Effect Of Jiushen Mixture On Renal Interstitial Fibrosis In Rat With Unilateral Ureter Obstruction

Objective:To study the effect of Jiushen mixture to renal interstitial fibrosis through observe body weight, kidney weight/body weight, Scr, Bun, NAG and the expression of TGF-β1,CTGF,Col,Col III,FN,TGF-β1mRNA,CTGFmRNA in rat with unilateral ureter obstruction.Methods:The healthy and clean SD rats were randomLy divided into seven groups, namely normal control, sham operation group, model group, benazepril group and save the Jiushen mixture of small, medium, large doses of a group. After the One week of the adaptability feeding, copy the UUO model, and then treatment for three weeks. Test weight, kidney weight/body weight, Scr, BUN, NAG, renal pathology, and take the experimental renal tissue, to detect the protein expression of TGF-β1, CTGF, Col, Col III, FN and TGF-β1mRNA, CTGF gene expression levels.Results:Compared with model group Jiushen Mixture treatment group's body weight was significantly increased (P<0.05), its to save the weight in the third weeks when compared with benazepril treatment group (P<0.05). kidney weight/body weight ratio reduced to varying degrees, in which to save the kidney mixture of small, medium dose of the most significant (P<0.01); save dose group than in the kidney mixture of benzene were significantly lower (P<0.05). The histopathology results show rescue renal mixture dose group, compared with model of renal tubular epithelial cell degeneration, interstitial inflammatory cell infiltration, proliferation of mesangial cells, renal interstitial fiber material proliferation were significantly reduced. Immunohistochemical detection of renal tissue prompted, save the tubular epithelial cells of the kidney mixture treatment group, compared to the intrinsic glomerular cells, TGF-β, CTGF and Col Ⅲ, Col Ⅲ, the expression of the FN with the model group significantly reduced. Renal tissue by RT-PCR detection of prompt rescue kidney mixture of each treatment group of TGF-β1mRNA, CTGF expression amount compared with the model group significantly with reducing (P<0.01), which rescue renal mixture rule large, medium dose group than the low dose group expression amount significantly with reducing (P<0.01), a dose-dependent relationship; inhibition of TGF-β1mRNA expression of kidney mixture dose group compared with the benazepril group to save a significant effect was excellent (P<0.01); inhibit CTGFmRNA expression and Benazepril treatment compared to save the kidney mixture of high-dose group a significant effect was excellent (P<0.01).Conclusion:Our study suggest that Jiushen Mixture has a certain protection to renal function and renal interstitial fibrosis. The mechanism may be inhibition of TGF-β1mRNA, CTGF mRNA, and reduce TGF-β1of CTGF expression in the renal tissueof, then Inhibit generation and accum-ulation of Col Ⅰ, Col Ⅲ, FN.