| Traditional Chinese medicine (TCMs), as the main therapia of ancient Chinese people, was the practical summary of clinical medication for thousands of years. However, the extensive source, as well as diverse methods of processing and reservation had result in the difficulty in quality control of TCMs. Thus, the quality control methods had become one of the critical issues in modernization of TCMs. In this work, the quality control and evaluation of three curcuma plants were studied based on metabonomics methods. The main contents were listed below:1) The metabolic profiling of curcuma wenyujin in diffirent developmental stages was studied based on GC-MS and HPLC metabonomics methods together with pattern recoginion. The resuluts indicated both GC-MS and HPLC metabolic pattern of C. wenyujin is similar to each other and disclosed the growth of C.wenyujin can be classified into four stages. In addition, the accumulation of four active components including germacrone,β-elemene, curcumin and curdione were determined by HPLC. The results indicated the content of four components is higer at September, but at that time, the dried weight is smallest. Considering the changes in dried weight, December and January is suitable time for collection, which is consist with the change in the metabolic patterns. This result can be used to make sure the best suitable harvest time of C.wenyujin and discriminate collection time of market medicine materials.2) GC-MS metabonomics was applied to study the metabolic profilings of C.wenyujin, C. phaeocaulis and C. kwangsiensis. The components of volatile oils of three curcuma plants were identified by virtue of mass library (NIST05) and Kovats index and were determined semi-quantitatively by normalization. The PCA and PLS-DA were used to construct the discrimination model of three curcuma plants. Four chemical markers, including curzerenone, germacrone, curdione and epicurzerenone were successfully selected to carry out quality control of market materials from three curcuma plants.3) The antioxidant capacities of different solvent extracts of three curcuma plants were studied. The dried and fresh herbs were extracted by acetic ether (EtOAc),95% ethanol (EtOH) and water, respectively. Then, these extracts were tested for their scavenging abilities to DPPH and OH free radical, chelating abilities to Fe2+, and reduction abilities to Fe3+. The results revealed that EtOAc and 95% EtOH extracts possess potent antioxidant activities against DPPH and OH free radical equal to positive control of Vitamin C and BHA. To screen out active components from the extracts, stepwise regression was used to construct the model of the antioxidant capacity via the content of components. Curcumine was then selected as effectively antioxidant activity components, which was consist with related references, suggesting that multivariate statistical analysis can be used to screen out active components.4) The antibacterial capacities of volatile oils from three curcuma plants against gram-positive bacteria (S. aureus, S.epidermidis and E. feacium) and Gram-negative bacteria (E. coli and P.aeruginosa) were determined by microdilution test. The results showed that both of the volatile oils from fresh and dried C.wenyujin are sensitive to all tested bacteria, and the antibacterial capacity of fresh oils is more significant than dried oils. The volatile oil from C. kwangsiensis had a lower minimal inhibitory concentration (MIC) than other oils. In addition, the antioxidant results revealed that volatile oils possess potent antioxidant activities against OH free radical equal to positive control of Vitamin C and BHA. However, the antioxidant capacity of volatile oil against other methods is not good.
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