Neuroanatomic Research Of Central Nucleus In The Regulation Of Mouse Renal Function

PARTâ… Role of Spinal Cord in Regulation Mouse Kidney:A Virally Mediated Trans-synaptic Tracing StudyTo determine the spinal innervation and neuronal connections is important for studying renal metabolic responses. In this study, the spinal cords of 13 adult male C57BL/6J strain mice were retrogradely mapped using injections of pseudorabies virus (PRV)-614. The virus injected into the kidney was specifically transported to the spinal cord. At 5d after injection of the PRV-614, PRV-614 positive cells were found in the intermediolateral cell column, the intercalates nucleus or the central autonomic nucleus of spinal cord segments T4 to L1, and most PRV-614 labeled cells were found in the T9 segment. Our results revealed neuroanatomic circuits between kidney and the spinal intermediolateral cell column (IML) neurons. PARTâ…¡Transneuronal Labeling of Brainstem Neuronal Populations by Injection of Pseudorabies Virus into Mice KidneysThe autonomic nervous system is the primary neural mediator of renal catabolic and anabolic responses to internal stimuli. Our understanding of the brainstem neuronal populations that project to kidney is important for studying renal metabolic responses. In this study, the spinal cords and brainstem of 19 adult male C57BL/6J strain mice were retrogradely mapped using injections of pseudorabies virus-614. The virus injected into the kidney was specifically transported to the spinal cord and brainstem through the renal nerve. The rostral ventromedial medulla, rostral ventrolateral medulla, medullary raphe nuclei, A5 region, Locus coeruleus and nucleus subcoeruleus all contributed to the regulation of innervating sympathetic activity within the kidney. Sympathetic outflow to the kidney was regulated by different types of neural populations, including serotonin-, tryptophan hydroxylase-, and tyrosine hydroxylase-positive neurons in the brainstem. Neural circuit tracing could reliably identify medullary neurons that received synaptic inputs originating from renal autonomic afferents. Our results reveal neuroanatomical substrates of the descending serotonergic and catecholaminergic neuronal circuits in the brainstem for the regulation of renal functions. Partâ…¢Laterodorsal Tegmentum and Pedunculopontine Tegmental Nucleus Circuits Regulate Renal Functions: Neuroanatomical Evidence in Mice ModelsNeurons in the laterodorsal tegmentum (LDTg) and pedunculopontine tegmental nucleus (PPTg) play important roles in central autonomic circuits of the kidney. In this study, we used a combination of retrograde tracers pseudorabies virus (PRV)-614 and fluorescence immunohistochemistry to characterize the neuroanatomic substrate of PPTg and LDTg innervating the kidney in the mouse. PRV-614-infected neurons were retrogradely labeled in the rostral and middle parts of LDTg, and the middle and caudal parts of PPTg after tracer injection in the kidney. PRV-614/TPH double-labeled neurons were mainly localized in the rostral of LDTg, whereas PRV-614/TH neurons were scattered within the three parts of LDTg. PRV-614/TPH and PRV-614/TH neurons were located predominantly in the caudal of PPTg (cPPTg). These data provided direct neuroanatomical foundation for the identification of serotonergic and catecholaminergic projections from the mid-brain tegmentum to the kidney.