Research Of Injury In Rat Skeletal Muscle Cells Stasis, Immune Mechanism

Purpose:The topic to be from apoptosis, immune cells the perspective of injury research stasis for the future from a clinical point of skeletal muscle apoptosis, immune injury blood stasis point of effective diagnosis and a reasonable grasp of different pathological cycle of drug use and provide experimental period room basis. Through exploration and research of this thesis, not only for the injury blood stasis syndrome make an important contribution to the objective, and more able to TCM Syndrome supplement the scientific content of the unity of the macro and micro, local and global unity, static and dynamic unity.Method:80 SD rats were randomly assigned into 8 groups, namely control group, 2h after the modeling group,8h after the modeling group,24h after the modeling group,2d,3d,5d,7d group after the model, n=10. Using sophisticated modeling of quantitative methods to combat gravity, the control group before modeling, and the remaining groups were as in the corresponding time points rats were killed, and isolated from skeletal muscle cells and blood tests apoptosis of skeletal muscle cells, pathological biopsy and blood indicators of immune cytokines IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-α, dynamic observation of apoptosis in skeletal muscle after injury, pathological states, changes in systemic immune through injury early stasis various objective of the experimental data to determine evidence of injury the nature of early blood stasis. Theory combined with traditional Chinese medicine diagnosis, through the systematic comparison of Chinese and Western medicine, Chinese medicine diagnostics to explore the macro and micro, local and global, static and dynamic unity of the theoretical basis.Results:①quantitative methods to combat the damage caused by blood stasis inevitably lead to accelerated apoptosis, and apoptosis was regular and over time the trend, and this law of non-linear in nature, with multiple levels of apoptosis progress and stability cycle.②higher positive rate by regulation of apoptosis protease caspase-3, confirmed that caspase-3 protease regulation of apoptosis between progress and stability in multiple cycles, and intrinsically linked with apoptosis.③by observing cytokine IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, found that apoptosis of blood stasis injury trends and immune cytokines dynamic equilibrium exists between the two-way coupling. The overall factor of immune cells start point, the overall surge in point, the overall decline of points, as long as the occurrence of systemic imbalance of cytokines, immune to the rapid reinforcement of stress, thereby stimulating cell apoptosis. The subject of immune cells found in consumption caused by systemic factors of apoptosis, which is a new scientific discovery, but also from a side reaction of the Chinese theory of yin and yang theory, and various cytokines and the ratio between TNF-αexample starting with TNF-αconfirmed the proportion of relevant indicators is possible too with less acceleration occurs leading to apoptosis, TNF-αis by no means a simple immune factors to promote target cell apoptosis.④HE staining by microscopy and further confirmed by blood, immune response, apoptosis, repair the linkage between the exact relationship, this relationship can be described clearly pathological cycle and apoptosis may explain the reason for the increase 4 times.⑤from the clinical significance of analysis, about a week, systemic immune response to reduce edema caused by cells generated righting drugs should be used; closed injury ideal treatment of early immune response is to reduce volatility.Conclusion: Injury there is blood stasis rats necrosis-like apoptosis, the underlying mechanism is a series of immune response.