Antioxidation Activity And Physical Function Of Fraction Polysaccharides Of Auricularia Auricula

Auricularia auricular is well known as a edible fungus which has highly nutritious and officinal function. Polysaccharides of Auricularia auricular(AAP) are main function components of Auricularia auricular and have various functions such as antitumor, modulating immunity, antifatigue, decreasing blood sugar and blood fat, antithrombus and so on.Preparation of fraction AAP and several functions including antioxidation in vitro and in vivo, anti irradiation and antifatigue, were studied in this paer. The main results of this paper as follows:1. The optimal processing condition of water-soluble AAP, which ratio of liquid to solid 102:1, extraction time 2.1h,extraction temperature 88℃, was gained by single factor experiments and response surface analysis method. The yield of water-soluble AAP was 15.1%.2. Acidity-soluble AAP and alkali-soluble AAP were prepared using phosphate buffer solution. The optimal elution conditions of purifying water-soluble AAP which were diameter-length ratio 1:30, eluent concentration 0.5mol/L, elution velocity 0.7mL/min, were obtained by single factor and orthogonal experiments.3. The ability of acidity-soluble fraction AAP(APG6), water-soluble AAP (AAPⅠ-1) and alkali-soluble fraction AAP(APG8) scavenging ABTS radical, DPPH radical, O2- radical, OH radical, H2O2, reducing power and restraining metal ion chelation and lipid peroxidation were studied. The results showed that APG8 have higher effect, APG6 lower than APG8, AAP I-1 little lower than APG6. The components with lower relative molecular weight of APG6 and APG8 had better antioxidation function.4. The antioxidation function in vivo of acidity-soluble AAP(APG6-3), AAP I-1 and alkali-soluble AAP(APG8-3) were studied. The results showed that APG8-3 have higher antioxidation function, APG6-3 little lower than APG8-3, AAPⅠ-1 lower than APG6-3. The antioxidation function of high dosage group obviously better than that of low dosage group. The results showed that fraction AAP with antioxidation in vitro had corresponding effect in vivo by correlation analysis between in vitro and in vivo.5. The anti irradiation function of APG6-3, AAPⅠ-1 and APG8-3 were studied. The results showed that oxidative damage caused by irradiation was main damage of irradiation. Every dosage group all had protection effect about immunity decreasing and oxidation damage caused by irradiation. APG6-3, AAPⅠ-1 and APG8-3 had its own advantage respectively, and the anti irriadation function of high dosage group obviously better than that of low dosage group. The function of anti irradiation of AAP was accomplished by fulfilling its antioxidation function. 6. The antifatigue function of APG6-3, AAPⅠ-1 and APG8-3 were studied. The results showed that fatigue caused by energy depletion, fatigue production blocking and oxygen free radical and lipid peroxidation reaction enhancement. APG8-3 had better function of prolonging forced-swimming time of mice, APG6-3 lower than APG8-3, AAPⅠ-1 lower than APG6-3; APG8-3 had better function of increasing LG and MG contents, AAPⅠ-1 than APG8-3, APG6-3 lower than AAPⅠ-1; APG8-3 had better function of decreasing BLA and BUN contents, AAPⅠ-1 and APG6-3 lower than APG8-3.To sum up, APG8-3 had better function than AAPⅠ-1 and APG6-3 about antioxidation in vitro and in vivo, anti irradiation and antifatigue, and AAPⅠ-1 and APG6-3 had approximate function. The function of anti irradiation and antifatigue of AAP was accomplished by fulfilling its antioxidation function. The results of this paper lay a foundation for AAP exploitation and application in medicine, health food, cosmetic and so on.